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Literature summary for 4.1.1.101 extracted from
- Purification and properties of malolactic enzyme from Lactobacillus murinus CNRZ 313 (1985), J. Appl. Biochem., 6, 374-383.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | at pH values substantially different from optimum pH 5.5, a positive cooperativity between substrate molecules is observed | Ligilactobacillus murinus | |
| 0.023 | - |
(S)-malate | 37°C, pH 5.5 | Ligilactobacillus murinus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | required, Km value 0.00014 mM | Ligilactobacillus murinus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
2 * 110000, SDS-PAGE | Ligilactobacillus murinus |
| 220000 | - |
gel filtration | Ligilactobacillus murinus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ligilactobacillus murinus | - |
- |
- |
| Ligilactobacillus murinus CNRZ 313 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ligilactobacillus murinus |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| presence of NAD+ protects against inactivation. Additiion of NAD+, after dissociation, restores malolactic activity | Ligilactobacillus murinus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate | - |
Ligilactobacillus murinus | (S)-lactate + CO2 | - |
? | |
| (S)-malate | - |
Ligilactobacillus murinus CNRZ 313 | (S)-lactate + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 110000, SDS-PAGE | Ligilactobacillus murinus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Ligilactobacillus murinus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
phosphate buffer | Ligilactobacillus murinus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | required, Km value 0.00045 mM | Ligilactobacillus murinus | |
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