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Literature summary for 4.1.1.101 extracted from
- Induction, purification and characterization of malolactic enzyme from Oenococcus oeni SD-2a (2014), Eur. Food Res. Technol., 239, 827-835.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12.5 | - |
(S)-malate | pH 6.0, 30°C | Oenococcus oeni |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | about 20% of the activity with Mn2+ | Oenococcus oeni | |
| Fe2+ | about 20% of the activity with Mn2+ | Oenococcus oeni | |
| Mg2+ | about 20% of the activity with Mn2+ | Oenococcus oeni | |
| Mn2+ | most effective divalent cation | Oenococcus oeni | |
| Zn2+ | about 20% of the activity with Mn2+ | Oenococcus oeni | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59000 | - |
x * 59000, mass spectrometry | Oenococcus oeni |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | 14% v/v, more than 50% of initial activity | Oenococcus oeni |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oenococcus oeni | - |
- |
- |
| Oenococcus oeni SD-2a | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oenococcus oeni |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 419.2 | - |
pH 6.0, 30°C | Oenococcus oeni |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, presence of 0.1 M NaCl, half-life 30 days | Oenococcus oeni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate | - |
Oenococcus oeni | (S)-lactate + CO2 | - |
? | |
| (S)-malate | - |
Oenococcus oeni SD-2a | (S)-lactate + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 59000, mass spectrometry | Oenococcus oeni |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Oenococcus oeni |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
50% residual activity | Oenococcus oeni |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Oenococcus oeni |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Oenococcus oeni | calculated | - |
4.8 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Oenococcus oeni | optimal conditions for expressions are presence of 7 g/l L-malic acid in the growth medium | up |
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