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Literature summary for 4.1.1.101 extracted from

  • Battermann, G.; Radler, F.
    A comparative study of malolactic enzyme and malic enzyme of different lactic acid bacteria (1991), Can. J. Microbiol., 37, 211-217.
No PubMed abstract available
Search for more literature for 4.1.1.101

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3
-
 L-malate pH 6.0, temperature not specified in the publication Oenococcus oeni
10.9
-
 L-malate pH 6.0, temperature not specified in the publication Leuconostoc mesenteroides

Metals/Ions

Metals/Ions Comment Organism Structure
K+ presence of K+ required, may be substituted by NH4+ Lacticaseibacillus casei
K+ presence of K+ required, may be substituted by NH4+ Leuconostoc mesenteroides
K+ presence of K+ required, may be substituted by NH4+ Oenococcus oeni
Mn2+ presence of Mn2+ required Lacticaseibacillus casei
Mn2+ presence of Mn2+ required, Km value 0.017 mM Oenococcus oeni
Mn2+ presence of Mn2+ required, Km value 0.1 mM Leuconostoc mesenteroides
NH4+ may substitute for K+ Lacticaseibacillus casei
NH4+ may substitute for K+ Leuconostoc mesenteroides
NH4+ may substitute for K+ Oenococcus oeni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 2 * 65000, SDS-PAGE Lacticaseibacillus casei
65000
-
 2 * 65000, SDS-PAGE Leuconostoc mesenteroides
65000
-
 2 * 65000, SDS-PAGE Oenococcus oeni
130000
-
 gel filtration Lacticaseibacillus casei
130000
-
 gel filtration Leuconostoc mesenteroides
130000
-
 gel filtration Oenococcus oeni

Organism

Organism UniProt Comment Textmining
Lacticaseibacillus casei
-
-
-
Leuconostoc mesenteroides
-
-
-
Leuconostoc mesenteroides B116
-
-
-
Oenococcus oeni
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lacticaseibacillus casei
-
 Leuconostoc mesenteroides
-
 Oenococcus oeni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-malate
-
 Lacticaseibacillus casei L-lactate + CO2
-
 ?
L-malate
-
 Leuconostoc mesenteroides L-lactate + CO2
-
 ?
L-malate
-
 Oenococcus oeni L-lactate + CO2
-
 ?
L-malate
-
 Leuconostoc mesenteroides B116 L-lactate + CO2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 65000, SDS-PAGE Lacticaseibacillus casei
dimer 2 * 65000, SDS-PAGE Leuconostoc mesenteroides
dimer 2 * 65000, SDS-PAGE Oenococcus oeni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8 6
-
 Lacticaseibacillus casei
5.8 6
-
 Leuconostoc mesenteroides
5.8 6
-
 Oenococcus oeni

Cofactor

Cofactor Comment Organism Structure
NAD+ presence of NAD+ required Lacticaseibacillus casei
NAD+ presence of NAD+ required Leuconostoc mesenteroides
NAD+ presence of NAD+ required Oenococcus oeni