Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Zymobacter palmae |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.15 M sodium citrate pH 5.5, 14% (w/v) PEG 3350 | Zymobacter palmae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on | Zymobacter palmae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59400 | - |
2 * 59400, calculated from amino acid sequence | Zymobacter palmae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | Zymobacter palmae | - |
acetaldehyde + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zymobacter palmae | Q8KTX6 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex 200 gel filtration | Zymobacter palmae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | - |
Zymobacter palmae | acetaldehyde + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 2 * 59400, calculated from amino acid sequence | Zymobacter palmae |
Synonyms | Comment | Organism |
---|---|---|
PDC | - |
Zymobacter palmae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Zymobacter palmae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
the enzyme retains 80% activity after incubation at 65°C for 30 min | Zymobacter palmae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | dependent on | Zymobacter palmae |