Application | Comment | Organism |
---|---|---|
synthesis | comparison of relevant properties for isobutanol production of Saccharomyces cerevisiae Aro10 and Lactococcus lactis KivD and KdcA genes. Activity in cell extracts reveals a superior Vmax/Km ratio of KdcA for alpha-ketoisovalerate and a wide range of linear and branched-chain 2-oxo acids. KdcA also shows the highest activity with pyruvate which, in engineered strains, can contribute to formation of ethanol as a by-product. During oxygen-limited incubation in the presence of glucose, strains expressing kdcA or kivD show a ca. twofold higher in vivo rate of conversion of alpha-ketoisovalerate into isobutanol than an Aro10-expressing strain. Cell extracts from cultures grown on different nitrogen sources reveal increased activity of constitutively expressed KdcA after growth on both valine and phenylalanine, while KivD and Aro10 activity is only increased after growth on phenylalanine | Lactococcus lactis subsp. lactis |
Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae, in a decarboxylase-negative pdc1Delta,pdc5Delta, pdc6Delta, aro10Delta background | Lactococcus lactis subsp. lactis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.42 | - |
2-oxoisocaproate | pH 7.0, 30°C | Lactococcus lactis subsp. lactis | |
7.73 | - |
2-oxoisovalerate | pH 7.0, 30°C | Lactococcus lactis subsp. lactis | |
12.9 | - |
2-oxomethylvalerate | pH 7.0, 30°C | Lactococcus lactis subsp. lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis subsp. lactis | Q684J7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoisocaproate | - |
Lactococcus lactis subsp. lactis | 3-methylbutanal + CO2 | - |
? | |
2-oxoisovalerate | - |
Lactococcus lactis subsp. lactis | 2-methylpropanal + CO2 | - |
? | |
2-oxomethylvalerate | - |
Lactococcus lactis subsp. lactis | pentanal + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KivD | - |
Lactococcus lactis subsp. lactis |