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Literature summary for 4.1.1.1 extracted from

  • Milne, N.; Van Maris, A.; Pronk, J.; Daran, J.
    Comparative assessment of native and heterologous 2-oxo acid decarboxylases for application in isobutanol production by Saccharomyces cerevisiae (2015), Biotechnol. Biofuels, 8, 204.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis comparison of relevant properties for isobutanol production of Saccharomyces cerevisiae Aro10 and Lactococcus lactis KivD and KdcA genes. Activity in cell extracts reveals a superior Vmax/Km ratio of KdcA for alpha-ketoisovalerate and a wide range of linear and branched-chain 2-oxo acids. KdcA also shows the highest activity with pyruvate which, in engineered strains, can contribute to formation of ethanol as a by-product. During oxygen-limited incubation in the presence of glucose, strains expressing kdcA or kivD show a ca. twofold higher in vivo rate of conversion of alpha-ketoisovalerate into isobutanol than an Aro10-expressing strain. Cell extracts from cultures grown on different nitrogen sources reveal increased activity of constitutively expressed KdcA after growth on both valine and phenylalanine, while KivD and Aro10 activity is only increased after growth on phenylalanine Lactococcus lactis subsp. lactis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae, in a decarboxylase-negative pdc1Delta,pdc5Delta, pdc6Delta, aro10Delta background Lactococcus lactis subsp. lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.42
-
2-oxoisocaproate pH 7.0, 30°C Lactococcus lactis subsp. lactis
7.73
-
2-oxoisovalerate pH 7.0, 30°C Lactococcus lactis subsp. lactis
12.9
-
2-oxomethylvalerate pH 7.0, 30°C Lactococcus lactis subsp. lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis subsp. lactis Q684J7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoisocaproate
-
Lactococcus lactis subsp. lactis 3-methylbutanal + CO2
-
?
2-oxoisovalerate
-
Lactococcus lactis subsp. lactis 2-methylpropanal + CO2
-
?
2-oxomethylvalerate
-
Lactococcus lactis subsp. lactis pentanal + CO2
-
?

Synonyms

Synonyms Comment Organism
KivD
-
Lactococcus lactis subsp. lactis