Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | lack of substrate activation in Neurospora crassa yeast pyruvate decarboxylase species | Neurospora crassa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetic analysis, overview. The v/[S] plots display negative cooperativity and hence deviate from Michaelis-Menten kinetics in just the opposite way | Neurospora crassa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Neurospora crassa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
- |
- |
Subunits | Comment | Organism |
---|---|---|
oligomer | the enzyme forms filamentous structures at pH 6.0-6.5 of octamers and dodecamers, NcPDC tetramers display the lowest catalytic efficiency among all functional oligomeric forms of this enzyme, analysis by analytical gel filtration, analytical ultracentrifugation and small-angle X-ray solution scattering | Neurospora crassa |
Synonyms | Comment | Organism |
---|---|---|
NcPDC | - |
Neurospora crassa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Neurospora crassa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.1 | - |
pyruvate | kcat per monomer, pH 7.0, 25°C | Neurospora crassa | |
12.5 | - |
pyruvate | kcat per monomer, pH 6.5, 25°C | Neurospora crassa | |
14 | - |
pyruvate | kcat per monomer, pH 6.0, MES-NaOH buffer, 25°C | Neurospora crassa | |
16.9 | - |
pyruvate | kcat per monomer, pH 5.5, 25°C | Neurospora crassa | |
17.2 | - |
pyruvate | kcat per monomer, pH 5.0, 25°C | Neurospora crassa | |
18.3 | - |
pyruvate | kcat per monomer, pH 6.0, phosphate buffer, 25°C | Neurospora crassa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | - |
Neurospora crassa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | pH-dependence overview | Neurospora crassa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Neurospora crassa |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme forms filamentous structures | Neurospora crassa |