Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NaCl | up to 3fold increase in activity at pH 5.4 | Lactococcus lactis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 1 mM, 95% inhibition | Lactococcus lactis | |
Cu2+ | 1 mM, 95% inhibition | Lactococcus lactis | |
ethylendiaminetetraacetate | 1 mM, 95% inhibition | Lactococcus lactis | |
p-hydroxymercuribenzoate | 1 mM, 40% inhibition | Lactococcus lactis | |
phosphoramidon | 1 mM, 48% inhibition | Lactococcus lactis | |
Zn2+ | 1 mM, 35% inhibition | Lactococcus lactis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
2-oxoisovalerate | pH 6.5, 37°C | Lactococcus lactis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Lactococcus lactis | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
4 * 61000, calculated, 4 * 66000, SDS-PAGE | Lactococcus lactis |
66000 | - |
4 * 61000, calculated, 4 * 66000, SDS-PAGE | Lactococcus lactis |
240000 | - |
gel filtration | Lactococcus lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | Q684J7 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
80.7 | - |
pH 6.5, 37°C | Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoisocaproate | 22.7% of the activity with 2-oxoisovalerate | Lactococcus lactis | 3-methylbutanal + CO2 | - |
? | |
2-oxoisovalerate | - |
Lactococcus lactis | 2-methylpropanal + CO2 | - |
? | |
2-oxomethylvalerate | 16.7% of the activity with 2-oxoisovalerate | Lactococcus lactis | ? | - |
? | |
3-phenylpyruvate | 8.8% of the activity with 2-oxoisovalerate | Lactococcus lactis | 2-phenylethanal + CO2 | - |
? | |
4-methylthio-2-oxobutanoate | 7.2% of the activity with 2-oxoisovalerate | Lactococcus lactis | 3-methylthiopropanal + CO2 | - |
? | |
indole-3-pyruvate | 0.1% of the activity with 2-oxoisovalerate | Lactococcus lactis | 2-(indol-3-yl)-ethanal + CO2 | - |
? | |
pyruvate | 0.6% of the activity with 2-oxoisovalerate | Lactococcus lactis | ethanal + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 61000, calculated, 4 * 66000, SDS-PAGE | Lactococcus lactis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Lactococcus lactis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
30 min, complete inactivation | Lactococcus lactis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Lactococcus lactis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | - |
Lactococcus lactis |