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Literature summary for 4.1.1.1 extracted from

  • Hiromasa, Y.; Aso, Y.; Yamashita, S.
    Purification of pyruvate dehydrogenase complex from an extreme thermophile, Bacillus caldolyticus, and its thermal stability (1993), Biosci. Biotechnol. Biochem., 57, 1062-1066.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.117
-
pyruvate
-
[Bacillus] caldolyticus
1.27
-
2-oxoisopentanoate
-
[Bacillus] caldolyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39800
-
1 * 39800 + 1 * 41700, the dimeric pyruvate decarboxylase is a component of the multienzyme complex pyruvate dehydrogenase, SDS-PAGE [Bacillus] caldolyticus
41700
-
1 * 39800 + 1 * 41700, the dimeric pyruvate decarboxylase is a component of the multienzyme complex pyruvate dehydrogenase, SDS-PAGE [Bacillus] caldolyticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information [Bacillus] caldolyticus pyruvate decarboxylase is one enzyme component of the pyruvate dehydrogenase complex ?
-
?

Organism

Organism UniProt Comment Textmining
[Bacillus] caldolyticus
-
pyruvate decarboxylase is one enzyme component of the pyruvate dehydrogenase complex
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
[Bacillus] caldolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-Oxoisopentanoate
-
[Bacillus] caldolyticus ?
-
?
additional information pyruvate decarboxylase is one enzyme component of the pyruvate dehydrogenase complex [Bacillus] caldolyticus ?
-
?
pyruvate
-
[Bacillus] caldolyticus acetaldehyde + CO2
-
?

Subunits

Subunits Comment Organism
dimer 1 * 39800 + 1 * 41700, the dimeric pyruvate decarboxylase is a component of the multienzyme complex pyruvate dehydrogenase, SDS-PAGE [Bacillus] caldolyticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
30 min, 50% loss of activity [Bacillus] caldolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
[Bacillus] caldolyticus