Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ketomalonate | activates | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
C221S | still possesses 20-30% specific activity compared to the wild type enzyme and can still be inhibited by the (E)-4-(4-chlorophenyl)-2-oxo-3-butenoic acid class of inhibitors/substrate analogues as well as cinnamaldehydes | Saccharomyces cerevisiae |
C222A | still possesses 20-30% specific activity compared to the wild type enzyme and can still be inhibited by the (E)-4-(4-chlorophenyl)-2-oxo-3-butenoic acid class of inhibitors/substrate analogues as well as cinnamaldehydes | Saccharomyces cerevisiae |
D28A | inactivated faster than the wild type enzyme | Saccharomyces cerevisiae |
E477Q | inactivated faster than the wild type enzyme | Saccharomyces cerevisiae |
H114F | inactivated faster than the wild type enzyme | Saccharomyces cerevisiae |
H115F | inactivated faster than the wild type enzyme | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(E)-4-(4-Chlorophenyl)-2-oxo-3-butenoic acid | wild type enzyme and mutant enzymes D28A, H114F, H115F and E477Q | Saccharomyces cerevisiae | |
glyoxalate | mechanism-based inhibitor | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | - |
Saccharomyces cerevisiae | acetaldehyde + CO2 | - |
? |