Protein Variants | Comment | Organism |
---|---|---|
D440E | active, but unlike the wild type enzyme, exhibits a lag phase in product formation which can be reduced by preincubation with 5 mM thiamine diphosphate. Mutant N467D shows decreased affinity for thiamine diphosphate | Zymomonas mobilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-hydroxypyruvate | - |
Zymomonas mobilis | |
phosphate | - |
Ipomoea batatas | |
phosphate | - |
Sarcina ventriculi | |
phosphate | - |
Zea mays | |
phosphate | - |
Zymomonas mobilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Zymomonas mobilis | |
0.5 | - |
pyruvate | - |
Zea mays | |
0.5 | - |
pyruvate | - |
Zymomonas mobilis | |
0.52 | - |
pyruvate | wild type enzyme and mutant enzyme E449D | Zymomonas mobilis | |
0.71 | - |
pyruvate | mutant enzyme H114Q | Zymomonas mobilis | |
0.86 | - |
pyruvate | mutant enzyme W487L | Zymomonas mobilis | |
0.95 | - |
pyruvate | mutant enzyme D440E and mutant enzyme N467D | Zymomonas mobilis | |
0.97 | - |
pyruvate | mutant enzyme F496I | Zymomonas mobilis | |
1.06 | - |
pyruvate | mutant enzyme F496H | Zymomonas mobilis | |
1.33 | - |
pyruvate | mutant enzyme V111A | Zymomonas mobilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Saccharomyces cerevisiae | |
Mg2+ | - |
Zea mays | |
Mg2+ | - |
Zymomonas mobilis | |
Mg2+ | - |
Ipomoea batatas | |
Mg2+ | - |
Saccharomyces uvarum | |
additional information | - |
Zymomonas mobilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pisum sativum | the enzyme catalyzes the penultimate step in alcohol fermentation | ? | - |
? | |
additional information | Zymomonas mobilis | the enzyme catalyzes the penultimate step in alcohol fermentation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetobacter sp. | - |
- |
- |
Aspergillus sp. | - |
- |
- |
Canavalia ensiformis | - |
- |
- |
Citrus sp. | - |
- |
- |
Clostridium botulinum | - |
- |
- |
Erwinia amylovora | - |
- |
- |
Hanseniaspora uvarum | - |
- |
- |
Ipomoea batatas | - |
- |
- |
Kluyveromyces sp. | - |
- |
- |
Neurospora crassa | - |
- |
- |
Pastinaca sativa | - |
- |
- |
Pisum sativum | - |
cv. Miko | - |
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces pastorianus | - |
- |
- |
Saccharomyces uvarum | - |
- |
- |
Sarcina ventriculi | - |
- |
- |
Schizosaccharomyces pombe | - |
- |
- |
Zea mays | - |
- |
- |
Zymomonas mobilis | - |
wild type and mutant enzymes E50D, E50Q, V111A, H113Q, H114Q, D440N, D440T, D440G, D440E, E449D, N467Q, N467D, W487L, F496I, F496H | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-Oxobutanoate | - |
Zymomonas mobilis | ? | - |
? | |
2-Oxopentanoate | - |
Zymomonas mobilis | ? | - |
? | |
3-Fluoropyruvate | decarboxylation is followed by release of F- | Saccharomyces cerevisiae | acetate + F- + CO2 | - |
? | |
3-Fluoropyruvate | decarboxylation is followed by release of F- | Zymomonas mobilis | acetate + F- + CO2 | - |
? | |
3-Hydroxypyruvate | - |
Zymomonas mobilis | Glycolaldehyde + CO2 | - |
? | |
additional information | the enzyme catalyzes the penultimate step in alcohol fermentation | Pisum sativum | ? | - |
? | |
additional information | the enzyme catalyzes the penultimate step in alcohol fermentation | Zymomonas mobilis | ? | - |
? | |
pyruvate | - |
Saccharomyces pastorianus | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Saccharomyces cerevisiae | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Neurospora crassa | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Pisum sativum | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Zea mays | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Schizosaccharomyces pombe | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Zymomonas mobilis | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Citrus sp. | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Acetobacter sp. | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Aspergillus sp. | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Canavalia ensiformis | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Clostridium botulinum | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Ipomoea batatas | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Saccharomyces uvarum | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Pastinaca sativa | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Kluyveromyces sp. | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Hanseniaspora uvarum | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Sarcina ventriculi | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Erwinia amylovora | acetaldehyde + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | - |
Saccharomyces pastorianus |
More | - |
Saccharomyces cerevisiae |
More | - |
Neurospora crassa |
More | - |
Zea mays |
More | - |
Sarcina ventriculi |
More | phosphate stabilizes the tetramer by shifting the dimer-tetramer equilibrium to higher pH values, without altering the conformation of the tetramer | Zymomonas mobilis |