Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | minimal effects on activity with the exception of a 35% activity decrease in Tris/HCl | Homo sapiens | |
CaCl2 | 1 mM, 40% decrease of activity in HEPES and a 15% decrease in Tris/HCl buffer | Homo sapiens | |
CuCl2 | 1 mM, almost completely inhibits enzyme activity | Homo sapiens | |
EDTA | 2 mM, negative effect on activity | Homo sapiens | |
MgCl2 | 1 mM, 10-20% decrease in all buffers except HEPES | Homo sapiens | |
additional information | removal of dithiothreitol from reaction results in a substantial loss of activity in both Tris/HCl and HEPES, a 40% reduction in bicine, and a 65% reduction in bis-Tris propane | Homo sapiens | |
NaCl | 100 mM, 30-60% decrease in activity. 50 mM, reductions of 10-25% of activity. 10 mM, 5-15% reduction of activity | Homo sapiens | |
ZnCl2 | 1 mM, almost completely inhibits enzyme activity. Biphasic curve with IC50 values of 0.025 and 0.49 mM, indicating that there may be two zinc binding sites in the enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
6,8-difluoromethylumbelliferyl phosphate | pH 8.0, 37°C, HEPES buffer (50 mM at pH 8.0) with 10 mM NaCl, 0.01% w/v Brij 35 and 0.5 mM DTT as additives | Homo sapiens | |
7.4 | - |
4-nitrophenylphosphate | pH 8.0, 37°C, HEPES buffer (50 mM at pH 8.0) with 10 mM NaCl, 0.01% w/v Brij 35 and 0.5 mM DTT as additives | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NRX4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenylphosphate + H2O | - |
Homo sapiens | 4-nitrophenol + phosphate | - |
? | |
6,8-difluoromethylumbelliferyl phosphate + H2O | - |
Homo sapiens | 6,8-difluoromethylumbelliferone + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PHPT1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Homo sapiens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | 37 | significantly more active at 37 °C than at 23°C | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
4-nitrophenylphosphate | pH 8.0, 37°C, HEPES buffer (50 mM at pH 8.0) with 10 mM NaCl, 0.01% w/v Brij 35 and 0.5 mM DTT as additives | Homo sapiens | |
0.39 | - |
6,8-difluoromethylumbelliferyl phosphate | pH 8.0, 37°C, HEPES buffer (50 mM at pH 8.0) with 10 mM NaCl, 0.01% w/v Brij 35 and 0.5 mM DTT as additives | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
good activity with Tris/HCl buffer, bicine buffer, bis-Tris propane buffer, and HEPES buffer. Bicine buffer and bis-Tris propane buffer giving slightly higher activity than Tris·HCl and bicine buufer | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
ZnCl2 | pH 8.0, 37°C. Biphasic curve with IC50 values of 0.025 and 0.49 mM, indicating that there may be two zinc binding sites in the enzyme | Homo sapiens | |
0.49 | - |
ZnCl2 | pH 8.0, 37°C. Biphasic curve with IC50 values of 0.025 and 0.49 mM, indicating that there may be two zinc binding sites in the enzyme | Homo sapiens | |
0.5 | - |
CuCl2 | pH 8.0, 37°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.047 | - |
4-nitrophenylphosphate | pH 8.0, 37°C, HEPES buffer (50 mM at pH 8.0) with 10 mM NaCl, 0.01% w/v Brij 35 and 0.5 mM DTT as additives | Homo sapiens | |
1.8 | - |
6,8-difluoromethylumbelliferyl phosphate | pH 8.0, 37°C, HEPES buffer (50 mM at pH 8.0) with 10 mM NaCl, 0.01% w/v Brij 35 and 0.5 mM DTT as additives | Homo sapiens |