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Literature summary for 3.9.1.3 extracted from

  • McCullough, B.S.; Barrios, A.M.
    Facile, fluorogenic assay for protein histidine phosphatase activity (2018), Biochemistry, 57, 2584-2589 .
    View publication on PubMed
Search for more literature for 3.9.1.3

Inhibitors

Inhibitors Comment Organism Structure
bovine serum albumin minimal effects on activity with the exception of a 35% activity decrease in Tris/HCl Homo sapiens
CaCl2 1 mM, 40% decrease of activity in HEPES and a 15% decrease in Tris/HCl buffer Homo sapiens
CuCl2 1 mM, almost completely inhibits enzyme activity Homo sapiens
EDTA 2 mM, negative effect on activity Homo sapiens
MgCl2 1 mM, 10-20% decrease in all buffers except HEPES Homo sapiens
additional information removal of dithiothreitol from reaction results in a substantial loss of activity in both Tris/HCl and HEPES, a 40% reduction in bicine, and a 65% reduction in bis-Tris propane Homo sapiens
NaCl 100 mM, 30-60% decrease in activity. 50 mM, reductions of 10-25% of activity. 10 mM, 5-15% reduction of activity Homo sapiens
ZnCl2 1 mM, almost completely inhibits enzyme activity. Biphasic curve with IC50 values of 0.025 and 0.49 mM, indicating that there may be two zinc binding sites in the enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
 6,8-difluoromethylumbelliferyl phosphate pH 8.0, 37°C, HEPES buffer (50 mM at pH 8.0) with 10 mM NaCl, 0.01% w/v Brij 35 and 0.5 mM DTT as additives Homo sapiens
7.4
-
 4-nitrophenylphosphate pH 8.0, 37°C, HEPES buffer (50 mM at pH 8.0) with 10 mM NaCl, 0.01% w/v Brij 35 and 0.5 mM DTT as additives Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NRX4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenylphosphate + H2O
-
 Homo sapiens 4-nitrophenol + phosphate
-
 ?
6,8-difluoromethylumbelliferyl phosphate + H2O
-
 Homo sapiens 6,8-difluoromethylumbelliferone + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
PHPT1
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Homo sapiens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
23 37 significantly more active at 37 °C than at 23°C Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.35
-
 4-nitrophenylphosphate pH 8.0, 37°C, HEPES buffer (50 mM at pH 8.0) with 10 mM NaCl, 0.01% w/v Brij 35 and 0.5 mM DTT as additives Homo sapiens
0.39
-
 6,8-difluoromethylumbelliferyl phosphate pH 8.0, 37°C, HEPES buffer (50 mM at pH 8.0) with 10 mM NaCl, 0.01% w/v Brij 35 and 0.5 mM DTT as additives Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 good activity with Tris/HCl buffer, bicine buffer, bis-Tris propane buffer, and HEPES buffer. Bicine buffer and bis-Tris propane buffer giving slightly higher activity than Tris·HCl and bicine buufer Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.025
-
 ZnCl2 pH 8.0, 37°C. Biphasic curve with IC50 values of 0.025 and 0.49 mM, indicating that there may be two zinc binding sites in the enzyme Homo sapiens
0.49
-
 ZnCl2 pH 8.0, 37°C. Biphasic curve with IC50 values of 0.025 and 0.49 mM, indicating that there may be two zinc binding sites in the enzyme Homo sapiens
0.5
-
 CuCl2 pH 8.0, 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.047
-
 4-nitrophenylphosphate pH 8.0, 37°C, HEPES buffer (50 mM at pH 8.0) with 10 mM NaCl, 0.01% w/v Brij 35 and 0.5 mM DTT as additives Homo sapiens
1.8
-
 6,8-difluoromethylumbelliferyl phosphate pH 8.0, 37°C, HEPES buffer (50 mM at pH 8.0) with 10 mM NaCl, 0.01% w/v Brij 35 and 0.5 mM DTT as additives Homo sapiens