Literature summary for 3.9.1.2 extracted from

Trentini, D.; Fuhrmann, J.; Mechtler, K.; Clausen, T.
Chasing phosphoarginine proteins: Development of a selective enrichment method using a phosphatase trap (2014), Mol. Cell. Proteomics, 13, 1953-1964.

Search for more literature for 3.9.1.2

Application

Application	Comment	Organism
analysis	development of a substrate-trapping mutant that retains binding affinity toward arginine-phosphorylated proteins but cannot hydrolyze the captured substrates. Mutant C9A stably binds to arginine-phosphorylated proteins. The substrate-trapping efficiency is improved by impeding the oligomerization of the phosphatase mutant. The engineered YwlE trap efficiently captures arginine-phosphorylated proteins from complex Bacillus subtilis ywlE- cell extracts, thus facilitating identification of phosphoarginine sites in the large pool of cellular protein modifications	Bacillus subtilis

Protein Variants

Protein Variants	Comment	Organism
C9A	substrate-trapping mutant, retains binding affinity toward arginine-phosphorylated proteins but cannot hydrolyze the captured substrates. Mutant C9A stably binds to arginine-phosphorylated proteins	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	P39155	-	-
Bacillus subtilis 168	P39155	-	-

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Release 2023.1 (January 2023)