Protein Variants | Comment | Organism |
---|---|---|
E232D | mutant enzyme binds the substrate analogue 4-methylbenzoyl-CoA more tightly than does the wild-type dehalogenase. The kcat for 4-chlorobenzoyl-CoS conversion to product is reduced 10000fold in the mutant. Increased sibstrate binding, decreased ring polarization, and decreased catalytic efficiency indicate that the repositioning of the point charge in the Glu232Arg mutant might affect the orientation of the Arg145 carboxylate with respect to the aromatic ring | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0037 | - |
4-chlorobenzoyl-CoA | pH 7.5, 25°C, wild-type enzyme | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-chlorobenzoyl-CoA + H2O | - |
Pseudomonas sp. | 4-hydroxybenzoyl-CoA + chloride | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00015 | - |
4-chlorobenzoyl-CoA | pH 7.5, 25°C, mutant enzyme E232G | Pseudomonas sp. | |
0.6 | - |
4-chlorobenzoyl-CoA | pH 7.5, 25°C, wild-type enzyme | Pseudomonas sp. |