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Literature summary for 3.8.1.6 extracted from

  • Muller, R.; Oltmanns, R.H.; Lingens, F.
    Enzymatic dehalogenation of 4-chlorobenzoate by extracts from Arthrobacter sp. SU DSM 20407 (1988), Biol. Chem. Hoppe-Seyler, 396, 57-571.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
H2O2 1mM, 20% activation Arthrobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
CuSO4 1 mM, 73.4% inhibition Arthrobacter sp.
ZnSO4 1 mM, 30.4% inhibition Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.056
-
4-Bromobenzoate
-
Arthrobacter sp.
0.086
-
4-Iodobenzoate
-
Arthrobacter sp.
0.12
-
4-Chlorobenzoate
-
Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
gel filtration Arthrobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-chlorobenzoate + H2O Arthrobacter sp.
-
4-hydroxybenzoate + Cl-
-
?
4-chlorobenzoate + H2O Arthrobacter sp. SU / DSM 20407
-
4-hydroxybenzoate + Cl-
-
?

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
-
-
Arthrobacter sp. SU / DSM 20407
-
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration, yield 5-10% Arthrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5
-
cell-free extracts Arthrobacter sp.

Storage Stability

Storage Stability Organism
room temperature, 4 h, 50%, 4°C, 20 h, 50%, -18°C, 2 weeks, 50% Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-bromobenzoate + H2O relative activity compared to 4-chlorobenzoate, 55% Arthrobacter sp. 4-hydroxybenzoate + Br-
-
?
4-bromobenzoate + H2O relative activity compared to 4-chlorobenzoate, 55% Arthrobacter sp. SU / DSM 20407 4-hydroxybenzoate + Br-
-
?
4-chlorobenzoate + H2O
-
Arthrobacter sp. 4-hydroxybenzoate + Cl-
-
?
4-chlorobenzoate + H2O
-
Arthrobacter sp. SU / DSM 20407 4-hydroxybenzoate + Cl-
-
?
4-iodobenzoate + H2O relative activity compared to 4-chlorobenzoate, 120% Arthrobacter sp. 4-hydroxybenzoate + I-
-
?
4-iodobenzoate + H2O relative activity compared to 4-chlorobenzoate, 120% Arthrobacter sp. SU / DSM 20407 4-hydroxybenzoate + I-
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
15
-
-
Arthrobacter sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 36
-
Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Arthrobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 8
-
Arthrobacter sp.