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Literature summary for 3.8.1.5 extracted from

  • Fortova, A.; Sebestova, E.; Stepankova, V.; Koudelakova, T.; Palkova, L.; Damborsky, J.; Chaloupkova, R.
    DspA from Strongylocentrotus purpuratus: The first biochemically characterized haloalkane dehalogenase of non-microbial origin (2013), Biochimie, 95, 2091-2096.
    View publication on PubMed

Application

Application Comment Organism
synthesis enzyme DspA is very enantioselective towards 2-bromobutane and may be attractive for biocatalysis Strongylocentrotus purpuratus

Cloned(Commentary)

Cloned (Comment) Organism
gene dspA, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Strongylocentrotus purpuratus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetic analysis, kinetic resolution of 2-bromobutane and 2-bromopentane Strongylocentrotus purpuratus
0.92
-
1,3-dibromopropane pH 8.6, 37°C, recombinant His-tagged enzyme Strongylocentrotus purpuratus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
DspA, with an expected molecular mass of 36 kDa, existed as a dimer in the absence of the salt, but is predominantly monomeric in the presence of 150 mM NaCl, native PAGE and gel filtration Strongylocentrotus purpuratus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Strongylocentrotus purpuratus the enzyme shows a preference for terminally brominated hydrocarbons and enantioselectivity towards beta-brominated alkanes ?
-
?

Organism

Organism UniProt Comment Textmining
Strongylocentrotus purpuratus
-
gene dspA
-

Purification (Commentary)

Purification (Comment) Organism
gene dspA, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity Strongylocentrotus purpuratus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(bromomethyl)cyclohexane + H2O
-
Strongylocentrotus purpuratus cyclohexylmethanol + bromide
-
?
1,2,3-tribromopropane + H2O
-
Strongylocentrotus purpuratus 2,3-dibromopropanol + bromide
-
?
1,2-dibromo-3-chloropropane + H2O
-
Strongylocentrotus purpuratus 2-bromo-3-chloropropanol + bromide
-
?
1,2-dibromoethane + H2O
-
Strongylocentrotus purpuratus 2-bromoethanol + bromide
-
?
1,2-dibromopropane + H2O
-
Strongylocentrotus purpuratus 2-bromopropanol + bromide
-
?
1,3-dibromopropane + H2O high activity Strongylocentrotus purpuratus 3-bromopropanol + bromide
-
?
1,3-diiodopropane + H2O
-
Strongylocentrotus purpuratus 3-iodopropanol + iodide
-
?
1,5-dichloropentane + H2O
-
Strongylocentrotus purpuratus 5-chloropentanol + chloride
-
?
1-bromo-2-chloroethane + H2O
-
Strongylocentrotus purpuratus 2-chloroethanol + bromide
-
?
1-bromo-2-chloroethane + H2O high activity Strongylocentrotus purpuratus 2-chloroethanol + bromide
-
?
1-bromo-3-chloropropane + H2O
-
Strongylocentrotus purpuratus 3-chloropropanol + bromide
-
?
1-bromobutane + H2O
-
Strongylocentrotus purpuratus 1-butanol + bromide
-
?
1-bromohexane + H2O best substrate Strongylocentrotus purpuratus 1-hexanol + bromide
-
?
1-chloro-2-bromoethane + H2O
-
Strongylocentrotus purpuratus 2-bromoethanol + chloride
-
?
1-iodobutane + H2O
-
Strongylocentrotus purpuratus butanol + iodide
-
?
1-iodohexane + H2O
-
Strongylocentrotus purpuratus hexanol + iodide
-
?
1-iodopropane + H2O
-
Strongylocentrotus purpuratus propanol + iodide
-
?
2,3-dichloropropene + H2O
-
Strongylocentrotus purpuratus ?
-
?
2-iodobutane + H2O
-
Strongylocentrotus purpuratus ?
-
?
3-chloro-2-methylprop-1-ene + H2O
-
Strongylocentrotus purpuratus ?
-
?
4-bromobutyronitrile + H2O
-
Strongylocentrotus purpuratus ?
-
?
chlorocyclopentane + H2O
-
Strongylocentrotus purpuratus cyclopentanol + chloride
-
?
additional information the enzyme shows a preference for terminally brominated hydrocarbons and enantioselectivity towards beta-brominated alkanes Strongylocentrotus purpuratus ?
-
?
additional information substrate specificity profile of DspA, overview. No activity with 1-chlorobutane, 1-chlorohexane, 1,2-dichloroethane, 1,3-dichloropropane, 1,2-dichloropropane, 1,2,3-trichloropropane, bis(2-chlorethyl)ether, chlorocyclohexane, and bromocyclohexane. Medium enantioselectivity is observed with 2-bromobutane and 2-bromopentane, while none or very low enantioselectivity wi observed with methyl 2-bromobutyrate and ethyl 2-bromobutyrate Strongylocentrotus purpuratus ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 36000, DspA, with an expected molecular mass of 36 kDa, existed as a dimer in the absence of the salt, but is predominantly monomeric in the presence of 150 mM NaCl, SDS-PAGE Strongylocentrotus purpuratus
monomer 1 * 36000, DspA, with an expected molecular mass of 36 kDa, existed as a dimer in the absence of the salt, but is predominantly monomeric in the presence of 150 mM NaCl, SDS-PAGE Strongylocentrotus purpuratus

Synonyms

Synonyms Comment Organism
DspA
-
Strongylocentrotus purpuratus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Strongylocentrotus purpuratus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.57
-
1,3-dibromopropane pH 8.6, 37°C, recombinant His-tagged enzyme Strongylocentrotus purpuratus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
assay at Strongylocentrotus purpuratus

General Information

General Information Comment Organism
evolution the enzyme belongs to the alpha/beta-hydrolase superfamily Strongylocentrotus purpuratus