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Literature summary for 3.8.1.5 extracted from

  • Biedermannova, L.; Prokop, Z.; Gora, A.; Chovancova, E.; Kovacs, M.; Damborsky, J.; Wade, R.C.
    A single mutation in a tunnel to the active site changes the mechanism and kinetics of product release in haloalkane dehalogenase LinB (2012), J. Biol. Chem., 287, 29062-29074.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.8.1.5

Protein Variants

Protein Variants Comment Organism
L177W naturally occuring mutation, the single mutation in a tunnel to the active site changes the mechanism and kinetics of product release in LinB. Interactions of the bromide ion with the tryptophan increase free energy barrier for its passage, causing the reaction mechanism change Sphingobium indicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state and transient kinetics, kinetics of bromide ion binding, overview. Two distinct binding phases that suggest a two-step mechanism of bromide ion binding Sphingobium indicum

Organism

Organism UniProt Comment Textmining
Sphingobium indicum
-
-
-
Sphingobium indicum UT26
-
-
-

Reaction

Reaction Comment Organism Reaction ID
1-haloalkane + H2O = a primary alcohol + halide reaction mechanism, overview. The bromide release is the rate-limiting step of the catalytic cycle, overview Sphingobium indicum
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-haloalkane + H2O
-
 Sphingobium indicum a primary alcohol + halide
-
 ?
1-haloalkane + H2O
-
 Sphingobium indicum UT26 a primary alcohol + halide
-
 ?

Subunits

Subunits Comment Organism
More structural modeling using crystal structure, PDB code 1MJ5, overview Sphingobium indicum

Synonyms

Synonyms Comment Organism
LinB
-
 Sphingobium indicum

General Information

General Information Comment Organism
malfunction the single mutation in a tunnel to the active site changes the mechanism and kinetics of product release in LinB. Interactions of the bromide ion with the tryptophan increase free energy barrier for its passage, causing the reaction mechanism change Sphingobium indicum