Application | Comment | Organism |
---|---|---|
synthesis | unique catalytic activity and structural stability of DbjA in a broad pH range, combined with high enantioselectivity with particular substrates, make this enzyme a very versatile biocatalyst | Bradyrhizobium japonicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bradyrhizobium japonicum | P59337 | - |
- |
Bradyrhizobium japonicum USDA 110 | P59337 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
1-haloalkane + H2O = a primary alcohol + halide | Asp103, Glu127 and His280 are involved in the catalytic reaction, and two H-bond donating residues, Asn38 and Trp104, are involved in stabilization of a halogen group of the substrate | Bradyrhizobium japonicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-iodohexane + H2O | - |
Bradyrhizobium japonicum | 1-hexanol + iodide | - |
? | |
1-iodohexane + H2O | - |
Bradyrhizobium japonicum USDA 110 | 1-hexanol + iodide | - |
? | |
2-bromobutane + H2O | - |
Bradyrhizobium japonicum | 2-butanol + bromide | - |
? | |
2-bromobutane + H2O | - |
Bradyrhizobium japonicum USDA 110 | 2-butanol + bromide | - |
? | |
2-bromopentane + H2O | - |
Bradyrhizobium japonicum | 2-pentanol + bromide | - |
? | |
2-bromopentane + H2O | - |
Bradyrhizobium japonicum USDA 110 | 2-pentanol + bromide | - |
? | |
ethyl 2-bromobutyrate + H2O | - |
Bradyrhizobium japonicum | ? | - |
? | |
ethyl 2-bromobutyrate + H2O | - |
Bradyrhizobium japonicum USDA 110 | ? | - |
? | |
methyl 2-bromopropionate + H2O | - |
Bradyrhizobium japonicum | ? | - |
? | |
additional information | the pH does not have any effect on enantiodiscriminination of DbjA, but a decrease in temperature results in significantly enhanced enantioselectivity | Bradyrhizobium japonicum | ? | - |
? | |
additional information | the pH does not have any effect on enantiodiscriminination of DbjA, but a decrease in temperature results in significantly enhanced enantioselectivity | Bradyrhizobium japonicum USDA 110 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | at pH 6.1-6.5, SDS-PAGE and gel filtration | Bradyrhizobium japonicum |
monomer | at pH 5.7-5.9, SDS-PAGE and gel filtration | Bradyrhizobium japonicum |
More | oligomerization of DbjA is strongly pH-dependent: monomer, dimer, tetramer and a high molecular weight cluster of the enzyme occur in solution at different pH conditions, overview | Bradyrhizobium japonicum |
multimer | in solution above pH 9.6 | Bradyrhizobium japonicum |
tetramer | - |
Bradyrhizobium japonicum |
Synonyms | Comment | Organism |
---|---|---|
DbjA | - |
Bradyrhizobium japonicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bradyrhizobium japonicum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 50 | highest activity | Bradyrhizobium japonicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
different oligomeric states of DbjA possess different thermal stabilities. The enzyme is most stable at 49.1°C and pH 6.5 as a dimer | Bradyrhizobium japonicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | 10.4 | - |
Bradyrhizobium japonicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 11 | DbjA activity decreases below pH 7.0 and above pH 10.4 with no activity detected below pH 5.0 and above pH 11.0 | Bradyrhizobium japonicum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
different oligomeric states of DbjA possess different thermal stabilities. The enzyme is most stable at 49.1°C and pH 6.5 as a dimer | Bradyrhizobium japonicum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bradyrhizobium japonicum | sequence calculation | - |
5.89 |