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Literature summary for 3.8.1.5 extracted from

  • Mazumdar, P.A.; Hulecki, J.C.; Cherney, M.M.; Garen, C.R.; James, M.N.
    X-ray crystal structure of Mycobacterium tuberculosis haloalkane dehalogenase Rv2579 (2008), Biochim. Biophys. Acta, 1784, 351-362.
    View publication on PubMed
Search for more literature for 3.8.1.5

Application

Application Comment Organism
environmental protection organisms producing this enzyme are of great interest as bioremediants, the enzyme has also been shown to have uses in combating chemical warfare where it can act against toxic agents like mustard gas Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment) Organism
the ORF of Rv2579 is inserted into the cloning vector pDONR-201, the resulting vector is used to transfer the coding sequence into the expression vector pDET-15 Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
the crystal structure of Rv2579 is determined at a resolution of 1.2 A, in addition, the structures in complex with 1,3-propanediol and 1,2-dichloroethane are determined Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-haloalkane + H2O Mycobacterium tuberculosis
-
 a primary alcohol + halide
-
 ?
1-haloalkane + H2O Mycobacterium tuberculosis H37Rv
-
 a primary alcohol + halide
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WMR9
-
-
Mycobacterium tuberculosis H37Rv P9WMR9
-
-

Purification (Commentary)

Purification (Comment) Organism
using a glutathione sepharose column and size-exclusion chromatography, the GST tag is removed by digestion with AcTEV protease Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dichloroethane + 2 H2O
-
 Mycobacterium tuberculosis 1,2-ethanediol + 2 chloride
-
 ?
1,2-dichloroethane + 2 H2O
-
 Mycobacterium tuberculosis H37Rv 1,2-ethanediol + 2 chloride
-
 ?
1,3-dibromopropane + 2 H2O
-
 Mycobacterium tuberculosis 1,3-propanediol + 2 bromide
-
 ?
1,3-dibromopropane + 2 H2O
-
 Mycobacterium tuberculosis H37Rv 1,3-propanediol + 2 bromide
-
 ?
1-haloalkane + H2O
-
 Mycobacterium tuberculosis a primary alcohol + halide
-
 ?
1-haloalkane + H2O
-
 Mycobacterium tuberculosis H37Rv a primary alcohol + halide
-
 ?

Synonyms

Synonyms Comment Organism
haloalkane dehalogenase
-
 Mycobacterium tuberculosis
Rv2579
-
 Mycobacterium tuberculosis