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Literature summary for 3.8.1.5 extracted from

  • Sato, Y.; Natsume, R.; Tsuda, M.; Damborsky, J.; Nagata, Y.; Senda, T.
    Crystallization and preliminary crystallographic analysis of a haloalkane dehalogenase, DbjA, from Bradyrhizobium japonicum USDA110 (2007), Acta Crystallogr. Sect. F, 63, 294-296.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
environmental protection haloalkane dehalogenases are key enzymes for the degradation of halogenated aliphatic pollutants Bradyrhizobium japonicum

Cloned(Commentary)

Cloned (Comment) Organism
the plasmid pYBJA2 is used for expression of the protein in Escherichia coli BL21 cells, the DbjA construct codes for residues 1-310 of DbjA with its C-terminus fused to a His6-tag Bradyrhizobium japonicum

Crystallization (Commentary)

Crystallization (Comment) Organism
the crystal diffracts to 1.75 A resolution Bradyrhizobium japonicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-haloalkane + H2O Bradyrhizobium japonicum
-
a primary alcohol + halide
-
?

Organism

Organism UniProt Comment Textmining
Bradyrhizobium japonicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using a Ni Chelating Sepharose Fast Flow column Bradyrhizobium japonicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-haloalkane + H2O
-
Bradyrhizobium japonicum a primary alcohol + halide
-
?
additional information DjA has a high catalytic activity for beta-methylated haloalkanes Bradyrhizobium japonicum ?
-
?

Synonyms

Synonyms Comment Organism
DbjA
-
Bradyrhizobium japonicum
haloalkane dehalogenase
-
Bradyrhizobium japonicum