Literature summary for 3.8.1.5 extracted from

Nakamura, T.; Zamocky, M.; Zdrahal, Z.; Chaloupkova, R.; Monincova, M.; Prokop, Z.; Nagata, Y.; Damborsky, J.
Expression of glycosylated haloalkane dehalogenase LinB in Pichia pastoris (2006), Protein Expr. Purif., 46, 85-91.

Search for more literature for 3.8.1.5

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Sphingomonas paucimobilis	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
31700	-	x * 31700, expression in Escherichia coli, x * 34800, expression in Pichia pastoris, SDS-PAGE. x * 33800, His-tagged enzyme expressed in Escherichia coli, mass spectrometry	Sphingomonas paucimobilis
33800	-	x * 31700, expression in Escherichia coli, x * 34800, expression in Pichia pastoris, SDS-PAGE. x * 33800, His-tagged enzyme expressed in Escherichia coli, mass spectrometry	Sphingomonas paucimobilis
34800	-	x * 31700, expression in Escherichia coli, x * 34800, expression in Pichia pastoris, SDS-PAGE. x * 33800, His-tagged enzyme expressed in Escherichia coli, mass spectrometry	Sphingomonas paucimobilis

Organism

Organism	UniProt	Comment	Textmining
Sphingomonas paucimobilis	P51698	expression in Pichia pastoris	-
Sphingomonas paucimobilis UT26	P51698	expression in Pichia pastoris	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	enzyme contains a 2.8 kDa high mannose core, glycosylation has no effect on activity, protein folding or thermal stability	Sphingomonas paucimobilis

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme expressed in Pichia pastoris	Sphingomonas paucimobilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.86	-	substrate 1-chlorobutane, 37°C, pH 8.6	Sphingomonas paucimobilis
15.6	-	substrate 1,2-dibromoethane, 37°C, pH 8.6	Sphingomonas paucimobilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1,2-dibromoethane + H2O	-	Sphingomonas paucimobilis	2-bromoethanol + bromide	-	?
1,2-dibromoethane + H2O	-	Sphingomonas paucimobilis UT26	2-bromoethanol + bromide	-	?
1-chlorobutane + H2O	-	Sphingomonas paucimobilis	butanol + chloride	-	r
1-chlorobutane + H2O	-	Sphingomonas paucimobilis UT26	butanol + chloride	-	r

Subunits

Subunits	Comment	Organism
?	x * 31700, expression in Escherichia coli, x * 34800, expression in Pichia pastoris, SDS-PAGE. x * 33800, His-tagged enzyme expressed in Escherichia coli, mass spectrometry	Sphingomonas paucimobilis

Synonyms

Synonyms	Comment	Organism
LinB	-	Sphingomonas paucimobilis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
41.7	-	melting temperature, enzyme expressed in Pichia pastoris	Sphingomonas paucimobilis
41.8	-	melting tempreature, enzyme expressed in Escherichia coli	Sphingomonas paucimobilis

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Release 2023.1 (January 2023)