Application | Comment | Organism |
---|---|---|
agriculture | biodegradation of 1,2-dichloropropane and 2-chloropropane via modified enzyme | Sphingomonas paucimobilis |
Cloned (Comment) | Organism |
---|---|
UT26, His-tagged protein expressed in Escherichia coli | Sphingomonas paucimobilis |
Protein Variants | Comment | Organism |
---|---|---|
F151W | UT26, His-tagged mutant | Sphingomonas paucimobilis |
W109L | UT26, His-tagged mutant, no activity | Sphingomonas paucimobilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.18 | - |
1-Chlorobutane | UT26, His-tagged purified protein | Sphingomonas paucimobilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3,4,6-tetrachloro-1,4,cyclohexadiene + 2 H2O | Sphingomonas paucimobilis | - |
2,5-dichloro-2,5-cyclohexadiene-1,4-diol + 2 chloride | - |
? | |
1,3,4,6-tetrachloro-1,4,cyclohexadiene + 2 H2O | Sphingomonas paucimobilis UT26 | - |
2,5-dichloro-2,5-cyclohexadiene-1,4-diol + 2 chloride | - |
? | |
n-haloalkanes + H2O | Sphingomonas paucimobilis | broad substrate range | primary alcohols + halide | - |
? | |
n-haloalkanes + H2O | Sphingomonas paucimobilis UT26 | broad substrate range | primary alcohols + halide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingomonas paucimobilis | - |
e.g. Pseudomonas paucimobilis | - |
Sphingomonas paucimobilis | - |
UT26 | - |
Sphingomonas paucimobilis UT26 | - |
UT26 | - |
Purification (Comment) | Organism |
---|---|
UT26, His-tagged protein purified on Ni-NTA resin | Sphingomonas paucimobilis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
1-haloalkane + H2O = a primary alcohol + halide | alpha/beta hydrolase with catalytic triad, i.e. nucleophile (Asp124)-histidine (H289)-acid (Asp260), during catalytic reaction | Sphingomonas paucimobilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
comparison of wild-type and His-tagged enzyme concerning reaction rates for several substrates, remaining activity about 80% | Sphingomonas paucimobilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3,4,6-tetrachloro-1,4,cyclohexadiene + 2 H2O | - |
Sphingomonas paucimobilis | 2,5-dichloro-2,5-cyclohexadiene-1,4-diol + 2 chloride | - |
? | |
1,3,4,6-tetrachloro-1,4,cyclohexadiene + 2 H2O | - |
Sphingomonas paucimobilis UT26 | 2,5-dichloro-2,5-cyclohexadiene-1,4-diol + 2 chloride | - |
? | |
1-chlorodecane + H2O | - |
Sphingomonas paucimobilis | decanol + chloride | - |
? | |
1-chlorodecane + H2O | - |
Sphingomonas paucimobilis UT26 | decanol + chloride | - |
? | |
2-chlorobutane + H2O | - |
Sphingomonas paucimobilis | 2-butanol + chloride | - |
? | |
2-chlorobutane + H2O | - |
Sphingomonas paucimobilis UT26 | 2-butanol + chloride | - |
? | |
haloalkane + H2O | - |
Sphingomonas paucimobilis | alcohol + halide | - |
? | |
haloalkane + H2O | - |
Sphingomonas paucimobilis UT26 | alcohol + halide | - |
? | |
n-haloalkanes + H2O | broad substrate range | Sphingomonas paucimobilis | primary alcohols + halide | - |
? | |
n-haloalkanes + H2O | broad substrate range | Sphingomonas paucimobilis UT26 | primary alcohols + halide | - |
? |