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Literature summary for 3.8.1.5 extracted from

  • Schanstra, J.P.; Ridder, I.S.; Heimeriks, G.J.; Rink, R.; Poelarends, G.J.; Kalk, K.H.; Dijkstra, B.W.; Janssen, D.B.
    Kinetic characterization and X-ray structure of a mutant of haloalkane dehalogenase with higher catalytic activity and modified substrate range (1996), Biochemistry, 35, 13185-13195.
No PubMed abstract available
Search for more literature for 3.8.1.5

Crystallization (Commentary)

Crystallization (Comment) Organism
GJ10, X-ray structure of F172W mutant Xanthobacter autotrophicus

Protein Variants

Protein Variants Comment Organism
F172W mutant with 10fold higher KM for 1-chlorohexane and 2fold higher KM for 1,2-dibromoethane, increased bromide release but velocity of hydrolysis of alkyl-enzyme bond remains unchanged Xanthobacter autotrophicus
F172Y mutant with 5fold higher KM for 1-chlorohexane Xanthobacter autotrophicus
additional information GJ10, several mutants of F172, tested for substrate binding Xanthobacter autotrophicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 1,2-Dichloroethane GJ10, calculated from rate constants for four-steps reaction mechanism Xanthobacter autotrophicus
0.008
-
 1,2-Dichloroethane GJ10, F172W mutant, calculated from rate constants for four-steps reaction mechanism Xanthobacter autotrophicus
4.3
-
 1,2-Dibromoethane GJ10, calculated from rate constants for four-steps reaction mechanism Xanthobacter autotrophicus
29
-
 1,2-Dibromoethane GJ10, F172W mutant, calculated from rate constants for four-steps reaction mechanism Xanthobacter autotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1,2-dichloroethane + H2O Xanthobacter autotrophicus
-
 2-chloroethanol + chloride
-
 ?
1,2-dichloroethane + H2O Xanthobacter autotrophicus GJ10
-
 2-chloroethanol + chloride
-
 ?

Organism

Organism UniProt Comment Textmining
Xanthobacter autotrophicus
-
 GJ10
-
Xanthobacter autotrophicus GJ10
-
 GJ10
-

Purification (Commentary)

Purification (Comment) Organism
GJ10 Xanthobacter autotrophicus

Reaction

Reaction Comment Organism Reaction ID
1-haloalkane + H2O = a primary alcohol + halide GJ10, four-step reaction mechanism with covalent ester intermediate Xanthobacter autotrophicus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dibromoethane + H2O
-
 Xanthobacter autotrophicus 2-bromoethanol + bromide
-
 ?
1,2-dibromoethane + H2O
-
 Xanthobacter autotrophicus GJ10 2-bromoethanol + bromide
-
 ?
1,2-dichloroethane + H2O
-
 Xanthobacter autotrophicus 2-chloroethanol + chloride
-
 ?
1,2-dichloroethane + H2O
-
 Xanthobacter autotrophicus GJ10 2-chloroethanol + chloride
-
 ?
1-chlorohexane + H2O
-
 Xanthobacter autotrophicus hexanol + chloride
-
 ?
1-chlorohexane + H2O
-
 Xanthobacter autotrophicus GJ10 hexanol + chloride
-
 ?
haloalkane + H2O
-
 Xanthobacter autotrophicus alcohol + halide
-
 ?
haloalkane + H2O
-
 Xanthobacter autotrophicus GJ10 alcohol + halide
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information GJ10, rate limiting step is hydrolysis of alkyl-enzyme intermediate Xanthobacter autotrophicus