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Literature summary for 3.8.1.5 extracted from
- Isolation and characterization of a haloalkane halidohydrolase from Rhodococcus erythropolis Y2 (1990), J. Gen. Microbiol., 136, 115-120.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | - |
Rhodococcus erythropolis |  |
| CuSO4 | - |
Rhodococcus erythropolis | |
| Dibromomethane | - |
Rhodococcus erythropolis | |
| Dichloromethane | - |
Rhodococcus erythropolis | |
| FeSO4 | - |
Rhodococcus erythropolis | |
| HgCl2 | - |
Rhodococcus erythropolis | |
| NEM | - |
Rhodococcus erythropolis | |
| NiSO4 | - |
Rhodococcus erythropolis | |
| ZnSO4 | - |
Rhodococcus erythropolis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.26 | - |
1-Chlorobutane | - |
Rhodococcus erythropolis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
gel filtration | Rhodococcus erythropolis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus erythropolis | - |
Y2 | - |
| Rhodococcus erythropolis Y2 | - |
Y2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| strain Y2 | Rhodococcus erythropolis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rhodococcus erythropolis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-chlorobutane + H2O | - |
Rhodococcus erythropolis | butanol + chloride | - |
? | |
| 1-chlorobutane + H2O | - |
Rhodococcus erythropolis Y2 | butanol + chloride | - |
? | |
| haloalkane + H2O | overview | Rhodococcus erythropolis | alcohol + halide | - |
? | |
| haloalkane + H2O | broad specificity | Rhodococcus erythropolis | alcohol + halide | - |
? | |
| haloalkane + H2O | highest activity towards alpha,omega disubstituted chloro-C2-C6 and bromo-C2-C6 alkanes and 4-chlorobutanol | Rhodococcus erythropolis | alcohol + halide | - |
? | |
| haloalkane + H2O | overview | Rhodococcus erythropolis Y2 | alcohol + halide | - |
? | |
| haloalkane + H2O | broad specificity | Rhodococcus erythropolis Y2 | alcohol + halide | - |
? | |
| haloalkane + H2O | highest activity towards alpha,omega disubstituted chloro-C2-C6 and bromo-C2-C6 alkanes and 4-chlorobutanol | Rhodococcus erythropolis Y2 | alcohol + halide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 34000, SDS-PAGE | Rhodococcus erythropolis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.2 | - |
- |
Rhodococcus erythropolis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 11 | more than 50% of maximal activity at pH 7.5 and 11.0 | Rhodococcus erythropolis |
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