Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.8.1.3 extracted from

  • Li, Y.; Yue, Y.; Zhang, H.; Yang, Z.; Wang, H.; Tian, S.; Wang, J.B.; Zhang, Q.; Wang, W.
    Harnessing fluoroacetate dehalogenase for defluorination of fluorocarboxylic acids in silico and in vitro approach (2019), Environ. Int., 131, 104999 .
    View publication on PubMed
Search for more literature for 3.8.1.3

Application

Application Comment Organism
degradation the enzyme has a great potential in lowing its energy barrier toward efluorination of per- or polyfluoropropionic acids. Future in silico and in vitro efforts focusing on the directed mutations and enzyme engineering are required to enable its efficient degradation toward perfluorocarboxylic acids Rhodopseudomonas palustris
environmental protection the enzyme has a great potential in lowing its energy barrier toward efluorination of per- or polyfluoropropionic acids. Future in silico and in vitro efforts focusing on the directed mutations and enzyme engineering are required to enable its efficient degradation toward perfluorocarboxylic acids Rhodopseudomonas palustris

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Rhodopseudomonas palustris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.12
-
 2-fluoropropionic acid pH 7.4, 30°C Rhodopseudomonas palustris

Organism

Organism UniProt Comment Textmining
Rhodopseudomonas palustris Q6NAM1
-
-
Rhodopseudomonas palustris ATCC BAA-98 Q6NAM1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3,3,3-tetrafluoropropionic acid + H2O
-
 Rhodopseudomonas palustris ?
-
 ?
2,3,3,3-tetrafluoropropionic acid + H2O
-
 Rhodopseudomonas palustris ATCC BAA-98 ?
-
 ?
2-fluoropropionic acid + H2O
-
 Rhodopseudomonas palustris 2-hydroxypropionate + fluoride
-
 ?
2-fluoropropionic acid + H2O
-
 Rhodopseudomonas palustris ATCC BAA-98 2-hydroxypropionate + fluoride
-
 ?

Synonyms

Synonyms Comment Organism
FAcD
-
 Rhodopseudomonas palustris
fluoroacetate dehalogenase
-
 Rhodopseudomonas palustris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Rhodopseudomonas palustris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.5
-
 2-fluoropropionic acid pH 7.4, 30°C Rhodopseudomonas palustris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Rhodopseudomonas palustris