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Literature summary for 3.8.1.3 extracted from

  • Ichiyama, S.; Kurihara, T.; Kogure, Y.; Tsunasawa, S.; Kawasaki, H.; Esaki, N.
    Reactivity of asparagine residue at the active site of the D105N mutant of fluoroacetate dehalogenase from Moraxella sp. B (2004), Biochim. Biophys. Acta, 1698, 27-36.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli Moraxella sp.

Protein Variants

Protein Variants Comment Organism
D105N site-directed mutagenesis, catalytic site mutant, shows reduced activity, but reactivation occurs due to hydrolytic deamination of the mutant Asn105 to the wild-type Asp105, reactivation in a time- and temperature-dependent manner Moraxella sp.

Organism

Organism UniProt Comment Textmining
Moraxella sp.
-
-
-
Moraxella sp. B
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from Escherichia coli Moraxella sp.

Reaction

Reaction Comment Organism Reaction ID
haloacetate + H2O = glycolate + halide As105 is a catalytic site residue functioning as a nucleophil attacking the alpha-carbon atom of the substrate to displace the fluorine atom, catalytic mechanism, possibly involving an imidate as intermediate Moraxella sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chloroacetate + H2O
-
Moraxella sp. glycolate + chloride
-
?
chloroacetate + H2O
-
Moraxella sp. B glycolate + chloride
-
?
fluoroacetate + H2O very high dissociation energy of the carbon-fluorine bond Moraxella sp. glycolate + fluoride
-
?
fluoroacetate + H2O very high dissociation energy of the carbon-fluorine bond Moraxella sp. B glycolate + fluoride
-
?

Synonyms

Synonyms Comment Organism
Fac-DEX
-
Moraxella sp.
fluoroacetate dehalogenase
-
Moraxella sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Moraxella sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
assay at Moraxella sp.