Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli | Moraxella sp. |
Protein Variants | Comment | Organism |
---|---|---|
D105N | site-directed mutagenesis, catalytic site mutant, shows reduced activity, but reactivation occurs due to hydrolytic deamination of the mutant Asn105 to the wild-type Asp105, reactivation in a time- and temperature-dependent manner | Moraxella sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moraxella sp. | - |
- |
- |
Moraxella sp. B | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli | Moraxella sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
haloacetate + H2O = glycolate + halide | As105 is a catalytic site residue functioning as a nucleophil attacking the alpha-carbon atom of the substrate to displace the fluorine atom, catalytic mechanism, possibly involving an imidate as intermediate | Moraxella sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chloroacetate + H2O | - |
Moraxella sp. | glycolate + chloride | - |
? | |
chloroacetate + H2O | - |
Moraxella sp. B | glycolate + chloride | - |
? | |
fluoroacetate + H2O | very high dissociation energy of the carbon-fluorine bond | Moraxella sp. | glycolate + fluoride | - |
? | |
fluoroacetate + H2O | very high dissociation energy of the carbon-fluorine bond | Moraxella sp. B | glycolate + fluoride | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Fac-DEX | - |
Moraxella sp. |
fluoroacetate dehalogenase | - |
Moraxella sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Moraxella sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
assay at | Moraxella sp. |