Literature summary for 3.8.1.3 extracted from

Kawasaki, H.; Miyoshi, K.; Tonomura, K.
Purification, crystallization and properties of haloacetate halidohydrolase from Pseudomonas species (1981), Agric. Biol. Chem., 45, 543-544.

No PubMed abstract available

Search for more literature for 3.8.1.3

Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Pseudomonas sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2	-	monofluoroacetate	-	Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
33000	-	2 * 33000, SDS-PAGE	Pseudomonas sp.
42000	-	gel filtration	Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
monofluoroacetate + H2O	Pseudomonas sp.	-	glycolate + HF	-	ir

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp.	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
H1	Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
38.4	-	crystallized	Pseudomonas sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
monofluoroacetate + H2O	-	Pseudomonas sp.	glycolate + HF	-	ir

Subunits

Subunits	Comment	Organism
dimer	2 * 33000, SDS-PAGE	Pseudomonas sp.

Synonyms

Synonyms	Comment	Organism
monohaloacetate dehalogenase	-	-

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Pseudomonas sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Pseudomonas sp.

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Release 2023.1 (January 2023)