Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, no activity is retained | Pseudomonas putida | |
Ca2+ | 1 mM, induced 3.4% decrease in alpha-helix, 4.4% increase in beta-sheet, respectively, with less than 25% activity loss in activity | Pseudomonas putida | |
Cu2+ | 1 mM, induces the transition of alpha-helix to beta-sheet and random coil at the same time with more than half of the enzymatic activity loss | Pseudomonas putida | |
Hg2+ | 1 mM, no activity is retained | Pseudomonas putida | |
additional information | little effects on alpha-helix and HadL AJ1 activity are observed with the presence of 1 mM Co2+ | Pseudomonas putida | |
Zn2+ | 1 mM, induced 5.1% decrease in alpha-helix, 5.4% increase in beta-sheet, respectively, with less than 25% activity loss in activity | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53800 | - |
gel filtration | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q52087 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-chloropropionate + H2O | - |
Pseudomonas putida | (R)-2-hydroxypropionic acid + chloride | - |
? | |
additional information | the alpha-helical structure of the enzyme plays a significant role during catalysis, while beta-sheet formation is a functional disadvantage. Analysis of environment-induced conformational and functional changes of 2-haloacid dehalogenase is informative for understanding the molecular basis of its stability and the design of the enzyme for future biotechnological applications in conjunction with its biochemical properties | Pseudomonas putida | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 26800, SDS-PAGE | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
HadL AJ1 | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Pseudomonas putida |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | 40°C: about 40% of maximal activity, 60°C: about 70% of maximal activity | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Pseudomonas putida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9 | 11 | pH 9.0: about 60% of maximal activity, pH 11.0: about 60% of maximal activity | Pseudomonas putida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | 24 h, structural and functional stability | Pseudomonas putida |