Literature summary for 3.8.1.2 extracted from

Adamu, A.; Wahab, R.A.; Shamsir, M.S.; Aliyu, F.; Huyop, F.
Deciphering the catalytic amino acid residues of l-2-haloacid dehalogenase (DehL) from Rhizobium sp. RC1 An in silico analysis (2017), Comput. Biol. Chem., 70, 125-132 .

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Protein Variants

Protein Variants	Comment	Organism
D13A	significant decrease in the free energy of binding for the DehL-L-2-chloropropionic acid model complex, indicating the involvement of the residue in catalysis and/or structural integrity of the active site	Rhizobium sp. RC1
H184A	significant decrease in the free energy of binding for the DehL-L-2-chloropropionic acid model complex, indicating the involvement of the residue in catalysis and/or structural integrity of the active site	Rhizobium sp. RC1
M48A	significant decrease in the free energy of binding for the DehL-L-2-chloropropionic acid model complex, indicating the involvement of the residue in catalysis and/or structural integrity of the active site	Rhizobium sp. RC1
R51A	significant decrease in the free energy of binding for the DehL-L-2-chloropropionic acid model complex, indicating the involvement of the residue in catalysis and/or structural integrity of the active site	Rhizobium sp. RC1
T17A	significant decrease in the free energy of binding for the DehL-L-2-chloropropionic acid model complex, indicating the involvement of the residue in catalysis and/or structural integrity of the active site	Rhizobium sp. RC1

Organism

Organism	UniProt	Comment	Textmining
Rhizobium sp. RC1	Q53187	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
(S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide	it is hypothesized that, in DehL, catalysis is initiated by a nucleophilic attack launched by the side-chain carboxyl of Asp13 on the C2 of L-2CP to form an ester intermediate, which is subsequently hydrolyzed by a water molecule activated by His184 followed by product release	Rhizobium sp. RC1	a

Synonyms

Synonyms	Comment	Organism
DehL	-	Rhizobium sp. RC1
L-2-haloacid dehalogenase	-	Rhizobium sp. RC1

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Release 2023.1 (January 2023)