Cloned (Comment) | Organism |
---|---|
gene dehII, DNA and amino acid sequence determination and analysis | Hymeniacidon perlevis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-bromopropionic acid + H2O | Hymeniacidon perlevis | - |
(R)-2-hydroxypropionic acid + bromide | - |
? | |
(S)-2-chloropropionic acid + H2O | Hymeniacidon perlevis | - |
(R)-2-hydroxypropionic acid + chloride | - |
? | |
iodoacetic acid + H2O | Hymeniacidon perlevis | - |
acetic acid + iodide + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hymeniacidon perlevis | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.06 | - |
substrate (S)-2-chloropropionic acid, pH 7.3, 30°C | Hymeniacidon perlevis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-bromopropionic acid + H2O | - |
Hymeniacidon perlevis | (R)-2-hydroxypropionic acid + bromide | - |
? | |
(S)-2-chloropropionic acid + H2O | - |
Hymeniacidon perlevis | (R)-2-hydroxypropionic acid + chloride | - |
? | |
(S)-2-chloropropionic acid + H2O | high activity | Hymeniacidon perlevis | (R)-2-hydroxypropionic acid + chloride | - |
? | |
iodoacetic acid + H2O | - |
Hymeniacidon perlevis | acetic acid + iodide + H+ | - |
? | |
iodoacetic acid + H2O | best substrate | Hymeniacidon perlevis | acetic acid + iodide + H+ | - |
? | |
additional information | no activity with 2-fluoropropionic acid | Hymeniacidon perlevis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DEH99 | - |
Hymeniacidon perlevis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Hymeniacidon perlevis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
assay at | Hymeniacidon perlevis |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the Group II family | Hymeniacidon perlevis |
physiological function | DEH99 is a (S)-2-haloacid dehalogenase, which can degrade (S)-2-chloropropionic acid, (S)-2-bromopropionic acid, and iodoacetic acid | Hymeniacidon perlevis |