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Literature summary for 3.8.1.2 extracted from
- Structure of a haloacid dehalogenase superfamily phosphatase PH1421 from Pyrococcus horikoshii OT3: oligomeric state and thermoadaptation mechanism (2008), Acta Crystallogr. Sect. D, 64, 1068-1077.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21-Codon Plus (DE3)-RIL cells | Pyrococcus horikoshii |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| oil-microbatch method, in 200 mM NaCl and 20 mM Tris-HCl pH 8.0, 25.5% (w/v) polyethylene glycol 4000, 0.17 M ammonium acetate, 15% (w/v) glycerol and 85 mM sodium acetate buffer, pH 4.6, at 20°C | Pyrococcus horikoshii |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dependent on | Pyrococcus horikoshii |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25600 | - |
SDS-PAGE | Pyrococcus horikoshii |
| 49700 | - |
calculated apparent molecular weight | Pyrococcus horikoshii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus horikoshii | - |
strain OT3 | - |
| Pyrococcus horikoshii OT-3 | - |
strain OT3 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HiPrep 26/10 column chromatography, Super Q Toyopearl 650M column chromatography, hydroxyapatite column chromatography, and Superdex 200 gel filtration | Pyrococcus horikoshii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | PH1421 is a magnesium-dependent haloacid dehalogenase phosphatase that acts on compounds closely related to 2-phosphoglycolate, the enzyme does possess 4-nitrophenyl phosphate hydrolase activity in the presence of Mg2+ | Pyrococcus horikoshii | ? | - |
? | |
| additional information | PH1421 is a magnesium-dependent haloacid dehalogenase phosphatase that acts on compounds closely related to 2-phosphoglycolate, the enzyme does possess 4-nitrophenyl phosphate hydrolase activity in the presence of Mg2+ | Pyrococcus horikoshii OT-3 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 25600, SDS-PAGE | Pyrococcus horikoshii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-2-haloacid dehalogenase | - |
Pyrococcus horikoshii |
| PH1421 | - |
Pyrococcus horikoshii |
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Release 2023.1 (January 2023)
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