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Literature summary for 3.8.1.2 extracted from

  • Barth, P.T.; Bolton, L.; Thomson, J.C.
    Cloning and partial sequencing of an operon encoding two Pseudomonas putida haloalkanoate dehalogenases of opposite stereospecificity (1992), J. Bacteriol., 174, 2612-2619.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.8.1.2

Application

Application Comment Organism
synthesis production of (S)-2-chloropropionic acid, which is used in the synthesis of optically active phenoxypropionic acid herbicides Pseudomonas putida

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and Pseudomonas putida hosts by using broad-host-range vectors Pseudomonas putida

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Pseudomonas putida 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33600
-
 calculated from amino acid sequence Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida
-
 AJ1
-
Pseudomonas putida AJ1
-
 AJ1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R,S)-2-chloropropionic acid + H2O
-
 Pseudomonas putida (S)-lactic acid + (S)-2-chloropropionic acid + HCl
-
 ?
(R,S)-2-chloropropionic acid + H2O
-
 Pseudomonas putida AJ1 (S)-lactic acid + (S)-2-chloropropionic acid + HCl
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Pseudomonas putida