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Literature summary for 3.8.1.2 extracted from

  • Mörsberger, F.M.; Müller, R.; Otto, M.K.; Lingens, F.; Kulbe, K.D.
    Purification and characterization of 2-halocarboxylic acid dehalogenase II from Pseudomonas spec. CBS 3 (1991), Biol. Chem. Hoppe-Seyler, 372, 915-922.
    View publication on PubMed
Search for more literature for 3.8.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
FeSO4 above 10 mM Pseudomonas sp.
mercury(II)-acetate
-
 Pseudomonas sp.
MnSO4 above 10 mM Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.57
-
 L-2-chloropropionate pH 9.5, 37°C Pseudomonas sp.
1.6
-
 Monochloroacetate pH 9.5, 37°C Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 2 * 29000 SDS-PAGE Pseudomonas sp.
64000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-2-haloalkanoic acid + H2O Pseudomonas sp.
-
 D-2-hydroxyalkanoic acid + halide
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
dithiothreitol increases enzyme activity slight Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. CBS3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.8
-
-
 Pseudomonas sp.

Storage Stability

Storage Stability Organism
50 mM Tris sulfate, pH 7.5 + 0.01% NaN3 at 25°C or -30°C, 30 days with remaining 60% activity Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-haloacid + H2O substrate specificity towards chlorinated and bromated substrates is limited to short chain monosubstituated 2-halocarboxylic acids, fluoro compounds are not converted Pseudomonas sp. (R)-2-hydroxyacid + halide
-
 ?
(S)-2-haloacid + H2O substrate specificity towards chlorinated and bromated substrates is limited to short chain monosubstituated 2-halocarboxylic acids, fluoro compounds are not converted Pseudomonas sp. CBS3 (R)-2-hydroxyacid + halide
-
 ?
2-bromopropionic acid + H2O
-
 Pseudomonas sp. ?
-
 ?
L-2-chloropropionate + H2O
-
 Pseudomonas sp. D-lactate + HCl
-
 ?
L-2-chloropropionate + H2O
-
 Pseudomonas sp. CBS3 D-lactate + HCl
-
 ?
L-2-chloropropionic acid + H2O
-
 Pseudomonas sp. D-2-hydroxypropionic acid + HCl
-
 ?
L-2-chloropropionic acid + H2O
-
 Pseudomonas sp. CBS3 D-2-hydroxypropionic acid + HCl
-
 ?
L-2-haloalkanoic acid + H2O
-
 Pseudomonas sp. D-2-hydroxyalkanoic acid + halide
-
 ?
monobromoacetate + H2O
-
 Pseudomonas sp. glycolate + HBr
-
 ?
monobromoacetate + H2O
-
 Pseudomonas sp. CBS3 glycolate + HBr
-
 ?
monochloroacetate + H2O
-
 Pseudomonas sp. glycolate + HCl
-
 ?
monochloroacetate + H2O
-
 Pseudomonas sp. CBS3 glycolate + HCl
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 29000 SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45
-
 Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Pseudomonas sp.

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas sp. isoelectric focusing
-
 6.2