Protein Variants | Comment | Organism |
---|---|---|
D180A | the mutation destabilizes the rotation of the nucleophile D10, fixes catalytic water around D10, and prevents K151 from approaching D10 | Pseudomonas sp. |
K151A | the mutant is almost inactive, the mutation destabilizes substrate orientation and affects the balance of the charge around the active site | Pseudomonas sp. |
K151R | the mutant is almost inactive | Pseudomonas sp. |
S118A | the mutant retains 30% of the activity of the wild type enzyme | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-2-chloropropionate + H2O | Pseudomonas sp. | - |
D-lactate + HCl | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-haloacid + H2O | - |
Pseudomonas sp. | (S)-2-hydroxyacid + halide | - |
? | |
(S)-2-haloacid + H2O | - |
Pseudomonas sp. | (R)-2-hydroxyacid + halide | - |
? | |
L-2-chloropropionate + H2O | - |
Pseudomonas sp. | D-lactate + HCl | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-2-haloacid dehalogenase | - |
Pseudomonas sp. |
L-DEX YL | - |
Pseudomonas sp. |