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Literature summary for 3.7.1.9 extracted from

  • Saku, T.; Fushinobu, S.; Jun, S.; Ikeda, N.; Nojiri, H.; Yamane, H.; Omori, T.; Wakagi, T.
    Purification, characterization, and steady-state kinetics of a meta-cleavage compound hydrolase from Pseudomonas fluorescens IP01 (2002), J. Biosci. Bioeng., 93, 568-574 .
    View publication on PubMed
Search for more literature for 3.7.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pseudomonas fluorescens

Protein Variants

Protein Variants Comment Organism
S103A the mutant has 100000fold lower activity than that of the wild type enzyme Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0073
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate 150 mM phosphate buffer, at pH 7.5 and 25°C Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate + H2O Pseudomonas fluorescens
-
 ?
-
 ?
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate + H2O Pseudomonas fluorescens IP01
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens P96965
-
-
Pseudomonas fluorescens IP01 P96965
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O slight hydrolytic activity Pseudomonas fluorescens ?
-
 ?
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O slight hydrolytic activity Pseudomonas fluorescens IP01 ?
-
 ?
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate + H2O
-
 Pseudomonas fluorescens ?
-
 ?
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate + H2O
-
 Pseudomonas fluorescens IP01 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 32000, SDS-PAGE Pseudomonas fluorescens

Synonyms

Synonyms Comment Organism
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate
-
 Pseudomonas fluorescens
6-isopropyl-HODA hydrolase
-
 Pseudomonas fluorescens
CumD
-
 Pseudomonas fluorescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate 150 mM phosphate buffer, at pH 7.5 and 25°C Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudomonas fluorescens

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 the enzyme activity is stable between pH 7.0 and 9.0 Pseudomonas fluorescens