Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | EDTA has no effect | Flavobacterium sp. |
Application | Comment | Organism |
---|---|---|
molecular biology | orf234, encoding an alpha/beta hydrolase, which is distantly related to the meta-fission product hydrolases such as XylF, PhnD, and CumD | Flavobacterium sp. |
Cloned (Comment) | Organism |
---|---|
overexpression as an N-terminal His-tag from pSM12 in Escherichia coli BL21(DE3), or from plasmid pSM13 in Pseudomonas aeruginosa PAO1161 | Flavobacterium sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DEPC | inhibits by 82% | Flavobacterium sp. | |
additional information | N-ethylmaleimide and p-chloromercuribenzoate show no significant effect | Flavobacterium sp. | |
PMSF | inhibits by 75% | Flavobacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0048 | - |
2-hydroxy-6-oxohepta-2,4-dienoate | - |
Flavobacterium sp. | |
0.0057 | - |
2-hydroxymuconic semialdehyde | - |
Flavobacterium sp. | |
0.0074 | - |
2-hydroxy-5-methylmuconic semialdehyde | - |
Flavobacterium sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Fe2+, Ca2+, Cu2+, Mg2+, Mn2+, Co2+, Li2+, and Zn2+ have no effect | Flavobacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
4 * 30000, SDS-PAGE | Flavobacterium sp. |
120000 | - |
gel filtration | Flavobacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flavobacterium sp. | - |
ATCC27551 | - |
Flavobacterium sp. ATCC 27551 | - |
ATCC27551 | - |
Purification (Comment) | Organism |
---|---|
by affinity chromatography | Flavobacterium sp. |
Storage Stability | Organism |
---|---|
-20°C, 20% glycerol, 15 days | Flavobacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxy-5-methylmuconic semialdehyde + H2O | - |
Flavobacterium sp. | ? | - |
? | |
2-hydroxy-5-methylmuconic semialdehyde + H2O | - |
Flavobacterium sp. ATCC 27551 | ? | - |
? | |
2-hydroxy-6-oxohepta-2,4-dienoate + H2O | - |
Flavobacterium sp. | ? | - |
? | |
2-hydroxy-6-oxohepta-2,4-dienoate + H2O | - |
Flavobacterium sp. ATCC 27551 | ? | - |
? | |
2-hydroxymuconic semialdehyde + H2O | - |
Flavobacterium sp. | ? | - |
? | |
2-hydroxymuconic semialdehyde + H2O | - |
Flavobacterium sp. ATCC 27551 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 30000, SDS-PAGE | Flavobacterium sp. |
Synonyms | Comment | Organism |
---|---|---|
meta-cleavage product hydrolase | - |
Flavobacterium sp. |
MfhA | - |
Flavobacterium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
loses just 20% activity after incubation for 2 hours | Flavobacterium sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 85 | above 75°C enzyme activity declines rapidly | Flavobacterium sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16 | - |
2-hydroxy-5-methylmuconic semialdehyde | - |
Flavobacterium sp. | |
42 | - |
2-hydroxymuconic semialdehyde | - |
Flavobacterium sp. | |
57 | - |
2-hydroxy-6-oxohepta-2,4-dienoate | - |
Flavobacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Flavobacterium sp. |