Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | isoenzyme HODHI is inhibited, isoenzyme HODHII is activated | Cupriavidus necator |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Cupriavidus necator | |
Mn2+ | isoenzyme HODHI is inhibited, isoenzyme HODHII is activated | Cupriavidus necator | |
PCMB | - |
Cupriavidus necator | |
Zn2+ | - |
Cupriavidus necator |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
2-hydroxy-6-oxohepta-2,4-dienoate | isoenzyme HODHII | Cupriavidus necator | |
0.058 | - |
2-hydroxy-6-oxohepta-2,4-dienoate | isoenzyme HODHI | Cupriavidus necator | |
0.36 | - |
2-hydroxymuconic semialdehyde | isoenzyme HODHI | Cupriavidus necator | |
62 | - |
2-hydroxymuconic semialdehyde | isoenzyme HODHII | Cupriavidus necator |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34400 | - |
3 * 34400, isoenzyme HODHI, SDS-PAGE of 2-mercaptoethanol reduced enzyme | Cupriavidus necator |
36700 | - |
3 * 36700, isoenzyme HODHII, Alcaligenes eutrophus, SDS-PAGE of 2-mercaptoethanol reduced enzyme | Cupriavidus necator |
104000 | - |
sedimentation equilibrium analysis | Cupriavidus necator |
116000 | - |
isozyme HODHII, sedimentation equilibrium analysis | Cupriavidus necator |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
ATCC 17707 | - |
Purification (Comment) | Organism |
---|---|
ATCC 17707, strain 345, isozyme HODHII: TOL-like plasmid encoded enzyme, strain RA1012, isozyme HODHI: chromosomally-encoded enzyme | Cupriavidus necator |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.1 | - |
isoenzyme HODHII | Cupriavidus necator |
17.4 | - |
isoenzyme HODHI | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxy-5-methyl-6-oxohepta-2,4-dienoate + H2O | no activity with isoenzyme HODHI, 41% of the activity with 2-hydroxy-6-oxohepta-2,4-dienoate, isoenzyme HODHII | Cupriavidus necator | ? | - |
? | |
2-hydroxy-5-methyl-muconic semialdehyde + H2O | 3% of the activity with 2-hydroxy-6-oxohepta-2,4-dienoate, isoenzyme HODHI. 5% of the activity with 2-hydroxy-6-oxohepta-2,4-dienoate, isoenzyme HODHI | Cupriavidus necator | ? | - |
? | |
2-hydroxy-6-oxohepta-2,4-dienoate + H2O | - |
Cupriavidus necator | ? | - |
? | |
2-hydroxy-7-methyl-6-oxoocta-2,4-dienoate + H2O | 149% of the activity with 2-hydroxy-6-oxohepta-2,4-dienoate, isoenzyme HODHI, no activity with isoenzyme HODHII | Cupriavidus necator | ? | - |
? | |
2-hydroxymuconate semialdehyde + H2O | 31% of the activity with 2-hydroxy-6-oxohepta-2,4-dienoate, isoenzyme HODHI. 12% of the activity with 2-hydroxy-6-oxohepta-2,4-dienoate, isoenzyme HODHII | Cupriavidus necator | formate + 2-oxopent-4-enoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 34400, isoenzyme HODHI, SDS-PAGE of 2-mercaptoethanol reduced enzyme | Cupriavidus necator |
trimer | 3 * 36700, isoenzyme HODHII, Alcaligenes eutrophus, SDS-PAGE of 2-mercaptoethanol reduced enzyme | Cupriavidus necator |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
isoenzyme HODHII is inactivated after 2 min, isoenzyme HODHI is inactivated after 4 min | Cupriavidus necator |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
- |
Cupriavidus necator |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | pH 7.6: 75-80% of the activity at pH 8.6, pH 9.0: marked decrease in activity above pH 9.0, similar pH profile of both isoenzymes | Cupriavidus necator |