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Literature summary for 3.7.1.8 extracted from

  • Kuatsjah, E.; Chan, A.; Hurst, T.; Snieckus, V.; Murphy, M.; Eltis, L.
    Metal- and serine-dependent meta-cleavage product hydrolases utilize similar nucleophile-activation strategies (2018), ACS Catal., 8, 11622-11632 .
No PubMed abstract available
Search for more literature for 3.7.1.8

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0033
-
 4,11-dicarboxy-8-hydroxy-9-methoxy-2-hydroxy-6-oxo-6-phenyl-hexa-2,4-dienoate pH 7, 25°C Sphingobium sp. SYK-6

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ Zn2+-dependent enzyme. 4,11-Dicarboxy-8-hydroxy-9-methoxy-2-hydroxy-6-oxo-6-phenyl-hexa-2,4-dienoate coordinates to the Zn2+ in a bidentate manner via the 1-carboxylate and 2-oxo groups Sphingobium sp. SYK-6

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4,11-dicarboxy-8-hydroxy-9-methoxy-2-hydroxy-6-oxo-6-phenyl-hexa-2,4-dienoate + H2O Sphingobium sp. SYK-6 the enzyme is involved in the catabolism of 2,2'-dihydroxy-3,3'-dimethoxy-5,5'-dicarboxybiphenyl, a lignin-derived biphenyl 5-carboxyvanillate + 4-carboxy-2-hydroxypenta-2,4-dienoate
-
 ?

Organism

Organism UniProt Comment Textmining
Sphingobium sp. SYK-6 G2IN02
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4,11-dicarboxy-8-hydroxy-9-methoxy-2-hydroxy-6-oxo-6-phenyl-hexa-2,4-dienoate + H2O the enzyme is involved in the catabolism of 2,2'-dihydroxy-3,3'-dimethoxy-5,5'-dicarboxybiphenyl, a lignin-derived biphenyl Sphingobium sp. SYK-6 5-carboxyvanillate + 4-carboxy-2-hydroxypenta-2,4-dienoate
-
 ?
4,11-dicarboxy-8-hydroxy-9-methoxy-2-hydroxy-6-oxo-6-phenyl-hexa-2,4-dienoate + H2O mechanism in which the metallocenter primarily catalyzes substrate tautomerization and the water required for the hydrolytic half-reaction is activated in a substrate-assisted manner Sphingobium sp. SYK-6 5-carboxyvanillate + 4-carboxy-2-hydroxypenta-2,4-dienoate
-
 ?

Synonyms

Synonyms Comment Organism
LigY
-
 Sphingobium sp. SYK-6

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.7
-
 4,11-dicarboxy-8-hydroxy-9-methoxy-2-hydroxy-6-oxo-6-phenyl-hexa-2,4-dienoate pH 7, 25°C Sphingobium sp. SYK-6

General Information

General Information Comment Organism
physiological function the enzyme is involved in the catabolism of 2,2'-dihydroxy-3,3'-dimethoxy-5,5'-dicarboxybiphenyl, a lignin-derived biphenyl Sphingobium sp. SYK-6