Literature summary for 3.7.1.8 extracted from

Lim, J.; Lee, J.; Lim, J.; Min, K.R.; Kim, C.; Kim, Y.
Characterization of the pcbD gene encoding 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase from Pseudomonas sp. P20 (2000), J. Microbiol. Biotechnol., 10, 258-263.

No PubMed abstract available

Search for more literature for 3.7.1.8

Application

Application	Comment	Organism
molecular biology	open reading frame corresponding to the pcbD gene consists of 855 base pairs with an ATG initiation codon and a TGA termination codon, able to encode a polypeptide with a molecular weight of 31732 containing 284 amino acid residues, deduced amino acid sequence has 62% identity with those of the HOPDA hydrolases of Pseudomonas putida KF715, P. pseudoalcaligenes KF707, and Burkholderia cepacia LB400, and also significant homology with those of other hydrolytic enzymes including esterase, transferase, and peptidase	Pseudomonas sp.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli HB101	Pseudomonas sp.

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp.	-	P20	-
Pseudomonas sp. P20	-	P20	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O	-	Pseudomonas sp.	benzoate + 2-hydroxypenta-2,4-dienoate	-	?
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O	-	Pseudomonas sp. P20	benzoate + 2-hydroxypenta-2,4-dienoate	-	?

Synonyms

Synonyms	Comment	Organism
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase	-	Pseudomonas sp.
HOPDA hydrolase	-	Pseudomonas sp.

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Release 2023.1 (January 2023)