BRENDA - Enzyme Database
show all sequences of 3.7.1.8

Comparative specificities of two evolutionarily divergent hydrolases involved in microbial degradation of polychlorinated biphenyls

Seah, S.Y.; Labbe, G.; Kaschabek, S.R.; Reifenrath, F.; Reineke, W.; Eltis, L.D.; J. Bacteriol. 183, 1511-1516 (2001) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Application
Application
Commentary
Organism
degradation
key determinant in the aerobic transformation of polychlorinated biphenyls by divergent biphenyl degraders
Rhodococcus globerulus
Cloned(Commentary)
Cloned (Commentary)
Organism
overexpression in Pseudomonas putida KT2442 containing vector pSS316
Rhodococcus globerulus
Inhibitors
Inhibitors
Commentary
Organism
Structure
(2E,4Z)-4-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
competetively inhibits the enzyme more effectively than (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
Rhodococcus globerulus
(2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Rhodococcus globerulus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00043
-
(2E,4E)-6-(3-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
0.00047
-
(2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
not chlorinated
Rhodococcus globerulus
0.00065
-
(2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
0.00075
-
(2E,4E)-6-(3,4-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
0.00104
-
(2E,4E)-6-(3,5-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
0.004
-
(2E,4Z)-4-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Rhodococcus globerulus
0.0044
-
(2E,4Z)-2,4-dihydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Rhodococcus globerulus
0.0069
-
(2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Rhodococcus globerulus
0.0129
-
(2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
0.018
-
(2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Rhodococcus globerulus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32580
-
by HPLC-ESMS analysis
Rhodococcus globerulus
Organism
Organism
UniProt
Commentary
Textmining
Rhodococcus globerulus
-
-
-
Rhodococcus globerulus P6
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
to more than 99% homogeneity
Rhodococcus globerulus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
13.2
-
purified enzyme
Rhodococcus globerulus
Storage Stability
Storage Stability
Organism
-80°C, 12 months
Rhodococcus globerulus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
4-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
(2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus P6
4-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
(2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
benzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
(2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus P6
benzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
(2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
2-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
(2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus P6
2-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
(2E,4E)-6-(3,4-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
3,4-dichlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
(2E,4E)-6-(3,4-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus P6
3,4-dichlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
(2E,4E)-6-(3,5-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
3,5-dichlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
(2E,4E)-6-(3,5-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus P6
3,5-dichlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
(2E,4E)-6-(3-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
3-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
(2E,4Z)-2,4-dihydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
benzoate + (2E)-2,4-dihydroxypenta-2,4-dienoic acid
-
-
-
?
(2E,4Z)-4-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
benzoate + (2E)-4-chloro-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
(2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
benzoate + (2E,4Z)-5-chloro-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
(2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
benzoate + (2Z)-3-chloro-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase
-
Rhodococcus globerulus
BphDP6
-
Rhodococcus globerulus
HOPDA hydrolase
-
Rhodococcus globerulus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0172
-
(2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Rhodococcus globerulus
1.03
-
(2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Rhodococcus globerulus
1.74
-
(2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
7.6
-
(2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
not chlorinated
Rhodococcus globerulus
11.2
-
(2E,4E)-6-(3,5-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
15.3
-
(2E,4E)-6-(3-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
29
-
(2E,4E)-6-(3,4-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
33.7
-
(2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
Application (protein specific)
Application
Commentary
Organism
degradation
key determinant in the aerobic transformation of polychlorinated biphenyls by divergent biphenyl degraders
Rhodococcus globerulus
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression in Pseudomonas putida KT2442 containing vector pSS316
Rhodococcus globerulus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
(2E,4Z)-4-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
competetively inhibits the enzyme more effectively than (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
Rhodococcus globerulus
(2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Rhodococcus globerulus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00043
-
(2E,4E)-6-(3-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
0.00047
-
(2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
not chlorinated
Rhodococcus globerulus
0.00065
-
(2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
0.00075
-
(2E,4E)-6-(3,4-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
0.00104
-
(2E,4E)-6-(3,5-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
0.004
-
(2E,4Z)-4-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Rhodococcus globerulus
0.0044
-
(2E,4Z)-2,4-dihydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Rhodococcus globerulus
0.0069
-
(2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Rhodococcus globerulus
0.0129
-
(2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
0.018
-
(2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Rhodococcus globerulus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32580
-
by HPLC-ESMS analysis
Rhodococcus globerulus
Purification (Commentary) (protein specific)
Commentary
Organism
to more than 99% homogeneity
Rhodococcus globerulus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
13.2
-
purified enzyme
Rhodococcus globerulus
Storage Stability (protein specific)
Storage Stability
Organism
-80°C, 12 months
Rhodococcus globerulus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
4-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
(2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus P6
4-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
(2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
benzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
(2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus P6
benzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
(2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
2-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
(2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus P6
2-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
(2E,4E)-6-(3,4-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
3,4-dichlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
(2E,4E)-6-(3,4-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus P6
3,4-dichlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
(2E,4E)-6-(3,5-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
3,5-dichlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
(2E,4E)-6-(3,5-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus P6
3,5-dichlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
(2E,4E)-6-(3-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
3-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
(2E,4Z)-2,4-dihydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
benzoate + (2E)-2,4-dihydroxypenta-2,4-dienoic acid
-
-
-
?
(2E,4Z)-4-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
benzoate + (2E)-4-chloro-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
(2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
benzoate + (2E,4Z)-5-chloro-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
(2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
benzoate + (2Z)-3-chloro-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0172
-
(2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Rhodococcus globerulus
1.03
-
(2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Rhodococcus globerulus
1.74
-
(2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
7.6
-
(2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
not chlorinated
Rhodococcus globerulus
11.2
-
(2E,4E)-6-(3,5-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
15.3
-
(2E,4E)-6-(3-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
29
-
(2E,4E)-6-(3,4-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
33.7
-
(2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
Other publictions for EC 3.7.1.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
755881
Jia
Identification and characteri ...
Arthrobacter sp. YC-RL1
Appl. Microbiol. Biotechnol.
103
6825-6836
2019
-
1
1
-
3
-
10
1
-
-
-
1
-
5
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1
-
-
-
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-
2
-
3
1
1
2
1
1
1
1
-
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-
1
1
-
-
3
-
-
10
-
1
-
-
-
1
-
-
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1
-
-
-
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2
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1
1
2
1
1
1
1
-
-
1
1
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-
755670
Kuatsjah
-
Metal- and serine-dependent m ...
Sphingobium sp. SYK-6
ACS Catal.
8
11622-11632
2018
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1
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1
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1
-
1
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-
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2
-
1
-
-
-
1
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
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-
1
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
757447
Dong
A water-assisted nucleophilic ...
Paraburkholderia xenovorans
J. Mol. Graph. Model.
76
448-455
2017
-
-
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-
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-
-
-
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1
-
1
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-
-
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-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
735243
Li
-
Insight into the catalytic mec ...
Paraburkholderia xenovorans
RSC Adv.
5
66591-66597
2015
-
-
-
1
-
-
-
-
-
-
-
1
-
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-
1
-
-
-
-
1
-
2
-
-
-
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-
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-
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Riddle
Purification and properties of ...
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Speare
Hammett analysis of a C-C hydr ...
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Seah
Identification of a serine hyd ...
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Lim
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Characterization of the pcbD g ...
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Genetic structures of the gene ...
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Location and sequence analysis ...
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Buenz
Purification of two isofunctio ...
Sphingomonas sp., Sphingomonas sp. RW1
Biodegradation
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Metabolism of 2,2'-dihydroxybi ...
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Purification and some properti ...
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