Crystallization (Comment) | Organism |
---|---|
wild-type, the S112C variant and S112C incubated with 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid by hanging drop vapor diffusion method at 20°C, to 1.6 A resolution, interaction between conserved active side residues and dienoate moiety of the substrate, the residue His265 is hydrogen-bonded to the 2-hydroxy/oxo substituent of the substrate, consistent with a role in catalyzing ketonization | Paraburkholderia xenovorans |
Protein Variants | Comment | Organism |
---|---|---|
S112C | lower enzyme activity | Paraburkholderia xenovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid | wild-type at 3.2°C | Paraburkholderia xenovorans | |
0.0028 | - |
2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid | S112C mutant at 25°C | Paraburkholderia xenovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paraburkholderia xenovorans | - |
LB400 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O | - |
Paraburkholderia xenovorans | benzoate + 2-hydroxypenta-2,4-dienoic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid hydrolase | - |
Paraburkholderia xenovorans |
BphD | - |
Paraburkholderia xenovorans |
HOPDA hydrolase | - |
Paraburkholderia xenovorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid | S112C mutant at 25°C | Paraburkholderia xenovorans | |
0.98 | - |
2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid | wild-type at 3.2°C | Paraburkholderia xenovorans | |
6.5 | - |
2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid | wild-type at 25°C | Paraburkholderia xenovorans |