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Literature summary for 3.7.1.8 extracted from
- Purification and some propertiees of a 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid hydrolyzing enzyme from Pseudomonas cruciviae S93 B1 involved in the degradation of biphenyl (1986), Agric. Biol. Chem., 50, 931-937.
No PubMed abstract available
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
x * 29000, SDS-PAGE of mercaptoethanol denatured enzyme | Pseudomonas cruciviae |
| 160000 | - |
gel filtration | Pseudomonas cruciviae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Pseudomonas cruciviae | enzyme is involved in the degradation of biphenyl | ? | - |
? |  |
| additional information | Pseudomonas cruciviae S93 B1 | enzyme is involved in the degradation of biphenyl | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas cruciviae | - |
S93 B1 | - |
| Pseudomonas cruciviae S93 B1 | - |
S93 B1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas cruciviae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 51.39 | - |
- |
Pseudomonas cruciviae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, pH 7.4, 10% loss of activity after 1 month | Pseudomonas cruciviae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-isopropylcatechol + H2O | - |
Pseudomonas cruciviae | ? | - |
? | |
| 3-isopropylcatechol + H2O | - |
Pseudomonas cruciviae S93 B1 | ? | - |
? | |
| 3-methylcatechol + H2O | - |
Pseudomonas cruciviae | ? | - |
? | |
| 3-methylcatechol + H2O | - |
Pseudomonas cruciviae S93 B1 | ? | - |
? | |
| biphenyl-2,3-diol + H2O | - |
Pseudomonas cruciviae | ? | - |
? | |
| biphenyl-2,3-diol + H2O | - |
Pseudomonas cruciviae S93 B1 | ? | - |
? | |
| catechol + H2O | - |
Pseudomonas cruciviae | ? | - |
? | |
| catechol + H2O | - |
Pseudomonas cruciviae S93 B1 | ? | - |
? | |
| additional information | enzyme is involved in the degradation of biphenyl | Pseudomonas cruciviae | ? | - |
? | |
| additional information | enzyme is involved in the degradation of biphenyl | Pseudomonas cruciviae S93 B1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 29000, SDS-PAGE of mercaptoethanol denatured enzyme | Pseudomonas cruciviae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
1 h, stable | Pseudomonas cruciviae |
| 60 | - |
pH 7.4, 5 min, 75% loss of activity | Pseudomonas cruciviae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Pseudomonas cruciviae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.7 | pH 6.5: about 65% of maximal activity, pH 8.7: about 25% of maximal activity | Pseudomonas cruciviae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 11 | 25°C, 6 h, stable | Pseudomonas cruciviae |
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