Application | Comment | Organism |
---|---|---|
industry | the improved catalytic efficiency of OPH expressed in recombinant Pichia pastoris makes it favorable for industrial applications | Sphingopyxis sp. |
Cloned (Comment) | Organism |
---|---|
gene oph, recombinant methano-inducible expression of the enzyme, ligated into the pPIC9K vector behind the alpha-factor signal sequence, in Pichia pastoris strain GS115 | Sphingopyxis sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | slight inhibition at 1 mM | Sphingopyxis sp. | |
Ca2+ | slight inhibition at 1 mM | Sphingopyxis sp. | |
Co2+ | slight inhibition at 1 mM | Sphingopyxis sp. | |
Cr2+ | moderate inhibition at 1 mM | Sphingopyxis sp. | |
Cu2+ | slight inhibition at 1 mM | Sphingopyxis sp. | |
Fe2+ | moderate inhibition at 1 mM | Sphingopyxis sp. | |
Hg2+ | strong inhibition at 1 mM | Sphingopyxis sp. | |
phenylmethanesulfonyl fluoride | strong inhibition | Sphingopyxis sp. | |
Sn2+ | slight inhibition at 1 mM | Sphingopyxis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics of the recombinant enzyme with 4-nitrophenyl acetate as substrate | Sphingopyxis sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | stimulates the enzyme at 1 mM | Sphingopyxis sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxidized polyvinyl alcohol + H2O | Sphingopyxis sp. | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingopyxis sp. | Q588Z2 | gene oph | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 1-67fold from Pichia pastoris strain GS115 culture supernatant by ultrafiltration, and hydrophobic interaction and cation exchange chromatography | Sphingopyxis sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
598 | - |
purified recombinant enzyme, substrate 4-nitrophenyl acetate, pH 8.0, 45°C | Sphingopyxis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxidized polyvinyl alcohol + H2O | - |
Sphingopyxis sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OPH | - |
Sphingopyxis sp. |
oxidized polyvinyl alcohol hydrolase | - |
Sphingopyxis sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
recombinant enzyme | Sphingopyxis sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
recombinant enzyme, stable up to | Sphingopyxis sp. |
47.5 | - |
recombinant enzyme, half-life is 5 h | Sphingopyxis sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant enzyme | Sphingopyxis sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | recombinant enzyme, stable at, over 90% activity remaning after 12 h, beyond this pH range, the OPH activity half-life is less than 1 h | Sphingopyxis sp. |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in degradation of polyvinyl alcohol | Sphingopyxis sp. |