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Literature summary for 3.7.1.7 extracted from

  • Yang, Y.; Zhang, D.; Liu, S.; Jia, D.; Du, G.; Chen, J.
    Expression and fermentation optimization of oxidized polyvinyl alcohol hydrolase in E. coli (2012), J. Ind. Microbiol. Biotechnol., 39, 99-104.
    View publication on PubMed

Application

Application Comment Organism
industry oxidized polyvinyl alcohol hydrolase, OPH, is a key enzyme in the degradation of PVA and might have a great potential for application in textile desizing processes Sphingopyxis sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression of the OPH gene lacking the sequence encoding the original signal peptide in Escherichia coli strain BL21 (DE3), the recombinant enzyme is secreted, productivity of recombinant OPH reaches 565.95 U/L/h without and 733.17 U/L/h in presence of 200 mM glycine. Subcloning in Escherichia coli strain JM109. Therecombinant extracellular enzyme inhibits growth of Escherichia coli cells Sphingopyxis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
x * 35000, recombinant OPH, SDS-PAGE Sphingopyxis sp.

Organism

Organism UniProt Comment Textmining
Sphingopyxis sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
Sphingopyxis sp. 4-nitrophenol + acetate
-
?

Subunits

Subunits Comment Organism
? x * 35000, recombinant OPH, SDS-PAGE Sphingopyxis sp.

Synonyms

Synonyms Comment Organism
OPH
-
Sphingopyxis sp.
oxidized polyvinyl alcohol hydrolase
-
Sphingopyxis sp.
oxidized PVA hydrolase
-
Sphingopyxis sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Sphingopyxis sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
assay at Sphingopyxis sp.

General Information

General Information Comment Organism
metabolism oxidized polyvinyl alcohol hydrolase is a key enzyme in the degradation of polyvinyl alcohol Sphingopyxis sp.