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Literature summary for 3.7.1.7 extracted from

  • Klomklang, W.; Tani, A.; Kimbara, K.; Mamoto, R.; Ueda, T.; Shimao, M.; Kawai, F.
    Biochemical and molecular characterization of a periplasmic hydrolase for oxidized polyvinyl alcohol from Sphingomonas sp. strain 113P3 (2005), Microbiology, 151, 1255-1262.
    View publication on PubMed
Search for more literature for 3.7.1.7

Application

Application Comment Organism
molecular biology exhibits 63% identity with OPH of Pseudomonas sp. strain VM15C and 29-32% identity with the polyhydroxybutyrate depolymerases from Mesorhizobium loti, Rhizobium sp. and Sinorhizobium meliloti Sphingomonas sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli under the control of the lac promoter Sphingomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ appreciably inhibits Sphingomonas sp.
additional information not inhibited by chelating agents, thiol agents and inhibitors of the respiratory chain Sphingomonas sp.
PMSF 1 mM strongly inhibits Sphingomonas sp.
Zn2+ appreciably inhibits Sphingomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 Oxidized polyvinyl alcohol
-
 Sphingomonas sp.
0.3
-
 p-nitrophenyl acetate
-
 Sphingomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Sphingomonas sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe3+ slightly activates Sphingomonas sp.
Mg2+ slightly activates Sphingomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 2 * 35000, SDS-PAGE Sphingomonas sp.
70000
-
 gel filtration Sphingomonas sp.

Organism

Organism UniProt Comment Textmining
Sphingomonas sp.
-
-
-
Sphingomonas sp. 113P3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, 5-9fold, gel filtration Sphingomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.1
-
 with oxidized polyvinyl alcohol as substrate Sphingomonas sp.
2.6
-
 with p-nitrophenyl acetate as substrate Sphingomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity on monoketones, diketones and esters Sphingomonas sp. ?
-
 ?
additional information no activity on monoketones, diketones and esters Sphingomonas sp. 113P3 ?
-
 ?
oxidized polyvinyl alcohol + H2O
-
 Sphingomonas sp. ?
-
 ?
oxidized polyvinyl alcohol + H2O
-
 Sphingomonas sp. 113P3 ?
-
 ?
p-nitrophenyl acetate + H2O
-
 Sphingomonas sp. ?
-
 ?
p-nitrophenyl acetate + H2O
-
 Sphingomonas sp. 113P3 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 35000, SDS-PAGE Sphingomonas sp.

Synonyms

Synonyms Comment Organism
OPH
-
 Sphingomonas sp.
oxidized polyvinyl alcohol hydrolase
-
 Sphingomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Sphingomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 stable for 30 min, 50 mM Tris/HCl buffer, pH 8.0 Sphingomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Sphingomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 8.5 50 mM sodium phosphate or Tris/HCl buffer Sphingomonas sp.