BRENDA - Enzyme Database show
show all sequences of 3.7.1.4

Prosequence of human lactase-phlorizin hydrolase modulates the folding of mature enzyme

Jacob, R.; Peters, K.; Naim, H.Y.; J. Biol. Chem. 277, 8217-8225 (2002)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
transient expression of mature enzyme and pro-enzyme in COS-1 cells, in vitro transcritption and translation
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Homo sapiens
5829
-
endoplasmic reticulum
the recombinant precusor in COS-1 cells resides in the ER, not mature wild-type
Homo sapiens
5783
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
mannose-rich, complex glycosylated mature enzyme, dependent on ooptimal folding of the processed proform in presence of the pro-peptide
Homo sapiens
proteolytic modification
enzyme is sythesized as a transport-competent precursor, i.e. pro-LPH, intracellular cleavage process in the trans-Golgi network between Arg734 and Leu 735 to yield the mature enzyme, i.e. LPHbetainitial, the propeptide of the zymogen, i.e. LPHalpha, modulates as a chaperone the folding of the mature enzyme after it has been cleaved off, mechanism, overview
Homo sapiens
Purification (Commentary)
Commentary
Organism
recombinant mature enzyme and pro-enzyme from COS-1 cells
Homo sapiens
Reaction
Reaction
Commentary
Organism
phloretin + H2O = phloretate + phloroglucinol
also hydrolyses other C-acetylated phenols related to phloretin
Homo sapiens
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
lactose + H2O
-
656110
Homo sapiens
beta-D-galactose + beta-D-glucose
-
-
-
?
additional information
the enzyme interacts with ER chaperones, immunglobulin binding protein, and calnexin at non-permissive temperatures in the ER
656110
Homo sapiens
?
-
-
-
-
phloretin + H2O
-
656110
Homo sapiens
phloretate + phloroglucinol
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
the mature enzyme shows a temperature-sensitive conformation, interaction with ER chaperones, immunglobulin binding protein, and calnexin, enzyme structure, overview
Homo sapiens
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
additional information
-
the mature enzyme shows a temperature-sensitive conformation
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
transient expression of mature enzyme and pro-enzyme in COS-1 cells, in vitro transcritption and translation
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Homo sapiens
5829
-
endoplasmic reticulum
the recombinant precusor in COS-1 cells resides in the ER, not mature wild-type
Homo sapiens
5783
-
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
mannose-rich, complex glycosylated mature enzyme, dependent on ooptimal folding of the processed proform in presence of the pro-peptide
Homo sapiens
proteolytic modification
enzyme is sythesized as a transport-competent precursor, i.e. pro-LPH, intracellular cleavage process in the trans-Golgi network between Arg734 and Leu 735 to yield the mature enzyme, i.e. LPHbetainitial, the propeptide of the zymogen, i.e. LPHalpha, modulates as a chaperone the folding of the mature enzyme after it has been cleaved off, mechanism, overview
Homo sapiens
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant mature enzyme and pro-enzyme from COS-1 cells
Homo sapiens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
lactose + H2O
-
656110
Homo sapiens
beta-D-galactose + beta-D-glucose
-
-
-
?
additional information
the enzyme interacts with ER chaperones, immunglobulin binding protein, and calnexin at non-permissive temperatures in the ER
656110
Homo sapiens
?
-
-
-
-
phloretin + H2O
-
656110
Homo sapiens
phloretate + phloroglucinol
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
the mature enzyme shows a temperature-sensitive conformation, interaction with ER chaperones, immunglobulin binding protein, and calnexin, enzyme structure, overview
Homo sapiens
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
additional information
-
the mature enzyme shows a temperature-sensitive conformation
Homo sapiens
Other publictions for EC 3.7.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750528
Han
Discovery and structural anal ...
Mycobacteroides abscessus 103, Mycobacteroides abscessus
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1
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1
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6
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1
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13
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6
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4
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1
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4
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6
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5
5
732550
Zhou
A comparative study on the met ...
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
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1
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1
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10
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2
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10
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1
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13
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2
2
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735304
Frank
-
Mutational analysis of the C-C ...
Topics Catal.
57
376-384
2014
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1
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3
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752294
Frank
-
Mutational analysis of the C- ...
Top. Catal.
57
376-384
2014
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1
1
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697434
Lehtimaeki
Adult-type hypolactasia is not ...
Homo sapiens
Clin. Sci.
115
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2008
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1
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699107
Delacour
Loss of galectin-3 impairs mem ...
Mus musculus 129Sv, Mus musculus
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701338
Cramm-Behrens
Apical cargo traverses endosom ...
Canis lupus familiaris
Traffic
9
2206-2220
2008
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2
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1
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1
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685892
Bulhoes
Correlation between lactose ab ...
Homo sapiens
Braz. J. Med. Biol. Res.
40
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2007
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1
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1
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1
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687838
Piepoli
Genotyping of the lactase-phlo ...
Homo sapiens
J. Biomol. Screen.
12
733-739
2007
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1
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1
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1
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1
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1
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1
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2
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673123
Bodlaj
Genotyping of the lactase-phlo ...
Homo sapiens
Clin. Chem.
52
148-151
2006
-
1
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1
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1
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1
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1
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3
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1
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3
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675574
Kuranuki
Dietary sucrose enhances intes ...
Rattus norvegicus
J. Nutr. Sci. Vitaminol.
52
347-351
2006
1
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1
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1
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675975
Bosse
Gata4 is essential for the mai ...
Mus musculus
Mol. Cell. Biol.
26
9060-9070
2006
3
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1
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1
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654284
Schoefer
Cloning and Expression of a Ph ...
, wK1
Appl. Environ. Microbiol.
70
6131-6137
2004
-
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1
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9
1
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1
3
3
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5
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1
1
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2
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11
2
1
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1
1
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1
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9
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1
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1
3
3
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1
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2
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11
2
1
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1
1
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-
-
-
2
2
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671823
Tseung
Partial amino acid sequence an ...
Homo sapiens, Oryctolagus cuniculus
Biochem. J.
380
211-218
2004
-
2
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675587
Verhave
Regulatory regions in the rat ...
Rattus norvegicus
J. Pediatr. Gastroenterol. Nutr.
39
275-285
2004
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1
1
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1
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656061
Mackey
Enzymatic hydrolysis of pyrido ...
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277
26858-26864
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6
3
3
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2
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2
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1
1
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2
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6
1
1
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2
1
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6
2
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3
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6
1
1
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1
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656110
Jacob
Prosequence of human lactase-p ...
Homo sapiens
J. Biol. Chem.
277
8217-8225
2002
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1
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1
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1
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208760
Ramaswamy
Lactase-phlorizin hydrolase co ...
Platyrrhini
Biochim. Biophys. Acta
403
446-455
1975
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2
3
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1
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1
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1
1
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12
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1
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1
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12
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1
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208761
Birkenmeier
Enzymatic properties of rat la ...
Rattus norvegicus
Biochim. Biophys. Acta
350
100-112
1974
7
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7
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1
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289291
Minamikawa
An inducible hydrolase from As ...
Aspergillus niger
Biochem. J.
116
889-897
1970
-
-
-
-
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12
2
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1
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1
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1
1
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12
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1
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1
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12
-
2
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1
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1
1
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12
-
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1
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1
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289292
Chatterjee
Metabolism of phloridzin by Er ...
Pantoea agglomerans, Pantoea agglomerans Y46
J. Bacteriol.
100
594-600
1969
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-
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2
1
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3
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1
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1
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1
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1
1
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