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Literature summary for 3.7.1.4 extracted from

  • Jacob, R.; Peters, K.; Naim, H.Y.
    Prosequence of human lactase-phlorizin hydrolase modulates the folding of mature enzyme (2002), J. Biol. Chem., 277, 8217-8225.
    View publication on PubMed
Search for more literature for 3.7.1.4

Cloned(Commentary)

Cloned (Comment) Organism
transient expression of mature enzyme and pro-enzyme in COS-1 cells, in vitro transcritption and translation Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-
endoplasmic reticulum the recombinant precusor in COS-1 cells resides in the ER, not mature wild-type Homo sapiens 5783
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein mannose-rich, complex glycosylated mature enzyme, dependent on ooptimal folding of the processed proform in presence of the pro-peptide Homo sapiens
proteolytic modification enzyme is sythesized as a transport-competent precursor, i.e. pro-LPH, intracellular cleavage process in the trans-Golgi network between Arg734 and Leu 735 to yield the mature enzyme, i.e. LPHbetainitial, the propeptide of the zymogen, i.e. LPHalpha, modulates as a chaperone the folding of the mature enzyme after it has been cleaved off, mechanism, overview Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant mature enzyme and pro-enzyme from COS-1 cells Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
phloretin + H2O = phloretate + phloroglucinol also hydrolyses other C-acetylated phenols related to phloretin Homo sapiens
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose + H2O
-
 Homo sapiens beta-D-galactose + beta-D-glucose
-
 ?
additional information the enzyme interacts with ER chaperones, immunglobulin binding protein, and calnexin at non-permissive temperatures in the ER Homo sapiens ?
-
 ?
phloretin + H2O
-
 Homo sapiens phloretate + phloroglucinol
-
 ?

Subunits

Subunits Comment Organism
More the mature enzyme shows a temperature-sensitive conformation, interaction with ER chaperones, immunglobulin binding protein, and calnexin, enzyme structure, overview Homo sapiens

Synonyms

Synonyms Comment Organism
lactase-phlorizin hydrolase
-
 Homo sapiens
LPH
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 the mature enzyme shows a temperature-sensitive conformation Homo sapiens