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Literature summary for 3.7.1.4 extracted from
- Enzymatic hydrolysis of pyridoxine-5'-beta-D-glucoside is catalyzed by intestinal lactase-phlorizin hydrolase (2002), J. Biol. Chem., 277, 26858-26864.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2',4'-dinitrophenyl-3-fluoro-2-deoxy-beta-D-glucopyranoside | specific 100fold inhibition of phloretin and lactose hydrolase activity, no inhibition of pyridoxine-5'-beta-D-glucoside hydrolase activity | Rattus norvegicus |  |
| beta-D-glucose | inhibition of pyridoxine-5'-beta-D-glucoside hydrolase activity | Rattus norvegicus | |
| cellobiose | inhibition of pyridoxine-5'-beta-D-glucoside hydrolase activity | Rattus norvegicus | |
| lactose | inhibition of pyridoxine-5'-beta-D-glucoside hydrolase activity, competitive | Rattus norvegicus | |
| maltose | inhibition of pyridoxine-5'-beta-D-glucoside hydrolase activity, inhibition is reduced to 60-75% by addition of 1-deoxynojirimycin | Rattus norvegicus | |
| additional information | no inhibition by pyridoxine, trehalose, and sucrose | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Rattus norvegicus | |
| 1 | - |
pyridoxine-5'-beta-D-glucoside | pH 6.0, 37°C | Rattus norvegicus | |
| 16 | - |
lactose | pH 6.0, 37°C | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| brush border | - |
Rattus norvegicus | 5903 | - |
| cytosol | - |
Rattus norvegicus | 5829 | - |
| membrane | - |
Rattus norvegicus | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phloretin + H2O | Rattus norvegicus | - |
phloretate + phloroglucinol | - |
? | |
| pyridoxine-5'-beta-D-glucoside + H2O | Rattus norvegicus | enzyme might play an important role in providing the bioavailability of pyridoxine-5'-beta-D-glucoside | pyridoxine + D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
dual specificity: phloretin hydrolase and pyridoxine-5'-beta-D-glucoside hydrolase activity | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 300fold from intestine brushh border membrane | Rattus norvegicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| phloretin + H2O = phloretate + phloroglucinol | also hydrolyses other C-acetylated phenols related to phloretin | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mucosa | - |
Rattus norvegicus | - |
| small intestine | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose + H2O | - |
Rattus norvegicus | beta-D-galactose + beta-D-glucose | - |
? | |
| additional information | the enzyme preparation also shows maltase-glucoamylase activity, EC 3.2.1.20, at 3.8 U/mg | Rattus norvegicus | ? | - |
? | |
| phloretin + H2O | - |
Rattus norvegicus | phloretate + phloroglucinol | - |
? | |
| pyridoxine-5'-beta-D-glucoside + H2O | - |
Rattus norvegicus | pyridoxine + D-glucose | - |
? | |
| pyridoxine-5'-beta-D-glucoside + H2O | enzyme might play an important role in providing the bioavailability of pyridoxine-5'-beta-D-glucoside | Rattus norvegicus | pyridoxine + D-glucose | - |
? | |
| pyridoxine-5'-beta-D-glucoside + lactose | - |
Rattus norvegicus | pyridoxine + beta-D-glucose + pyridoxine disaccharide | product identification, product is formed by a pyridoxine molecule with the 5'-beta-gkucoside and another glucose or galactose moiety | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 135000-140000, SDS-PAGE | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lactase phlorizin hydrolase | - |
Rattus norvegicus |
| lactase-phlorizin hydrolase | - |
Rattus norvegicus |
| LPH | - |
Rattus norvegicus |
| PNG hydrolase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
pyridoxine-5'-beta-D-glucoside | pH 6.0, 37°C | Rattus norvegicus | |
| 47 | - |
lactose | pH 6.0, 37°C | Rattus norvegicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Rattus norvegicus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 56 | - |
lactose | pH 6.0, 37°C | Rattus norvegicus | |
| 64 | - |
beta-D-glucose | pH 6.0, 37°C | Rattus norvegicus | |
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