BRENDA - Enzyme Database show
show all sequences of 3.7.1.4

Enzymatic hydrolysis of pyridoxine-5'-beta-D-glucoside is catalyzed by intestinal lactase-phlorizin hydrolase

Mackey, A.D.; Henderson, G.N.; Gregory, J.F., 3rd; J. Biol. Chem. 277, 26858-26864 (2002)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2',4'-dinitrophenyl-3-fluoro-2-deoxy-beta-D-glucopyranoside
specific 100fold inhibition of phloretin and lactose hydrolase activity, no inhibition of pyridoxine-5'-beta-D-glucoside hydrolase activity
Rattus norvegicus
beta-D-glucose
inhibition of pyridoxine-5'-beta-D-glucoside hydrolase activity
Rattus norvegicus
cellobiose
inhibition of pyridoxine-5'-beta-D-glucoside hydrolase activity
Rattus norvegicus
lactose
inhibition of pyridoxine-5'-beta-D-glucoside hydrolase activity, competitive
Rattus norvegicus
maltose
inhibition of pyridoxine-5'-beta-D-glucoside hydrolase activity, inhibition is reduced to 60-75% by addition of 1-deoxynojirimycin
Rattus norvegicus
additional information
no inhibition by pyridoxine, trehalose, and sucrose
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics
Rattus norvegicus
1
-
pyridoxine-5'-beta-D-glucoside
pH 6.0, 37°C
Rattus norvegicus
16
-
lactose
pH 6.0, 37°C
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
brush border
-
Rattus norvegicus
5903
-
cytosol
-
Rattus norvegicus
5829
-
membrane
-
Rattus norvegicus
16020
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
phloretin + H2O
Rattus norvegicus
-
phloretate + phloroglucinol
-
-
?
pyridoxine-5'-beta-D-glucoside + H2O
Rattus norvegicus
enzyme might play an important role in providing the bioavailability of pyridoxine-5'-beta-D-glucoside
pyridoxine + D-glucose
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
dual specificity: phloretin hydrolase and pyridoxine-5'-beta-D-glucoside hydrolase activity
-
Purification (Commentary)
Commentary
Organism
300fold from intestine brushh border membrane
Rattus norvegicus
Reaction
Reaction
Commentary
Organism
phloretin + H2O = phloretate + phloroglucinol
also hydrolyses other C-acetylated phenols related to phloretin
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mucosa
-
Rattus norvegicus
-
small intestine
-
Rattus norvegicus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
lactose + H2O
-
656061
Rattus norvegicus
beta-D-galactose + beta-D-glucose
-
-
-
?
additional information
the enzyme preparation also shows maltase-glucoamylase activity, EC 3.2.1.20, at 3.8 U/mg
656061
Rattus norvegicus
?
-
-
-
-
phloretin + H2O
-
656061
Rattus norvegicus
phloretate + phloroglucinol
-
-
-
?
pyridoxine-5'-beta-D-glucoside + H2O
-
656061
Rattus norvegicus
pyridoxine + D-glucose
-
-
-
?
pyridoxine-5'-beta-D-glucoside + H2O
enzyme might play an important role in providing the bioavailability of pyridoxine-5'-beta-D-glucoside
656061
Rattus norvegicus
pyridoxine + D-glucose
-
-
-
?
pyridoxine-5'-beta-D-glucoside + lactose
-
656061
Rattus norvegicus
pyridoxine + beta-D-glucose + pyridoxine disaccharide
product identification, product is formed by a pyridoxine molecule with the 5'-beta-gkucoside and another glucose or galactose moiety
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 135000-140000, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1
-
pyridoxine-5'-beta-D-glucoside
pH 6.0, 37°C
Rattus norvegicus
47
-
lactose
pH 6.0, 37°C
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
assay at
Rattus norvegicus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
56
-
lactose
pH 6.0, 37°C
Rattus norvegicus
64
-
beta-D-glucose
pH 6.0, 37°C
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2',4'-dinitrophenyl-3-fluoro-2-deoxy-beta-D-glucopyranoside
specific 100fold inhibition of phloretin and lactose hydrolase activity, no inhibition of pyridoxine-5'-beta-D-glucoside hydrolase activity
Rattus norvegicus
beta-D-glucose
inhibition of pyridoxine-5'-beta-D-glucoside hydrolase activity
Rattus norvegicus
cellobiose
inhibition of pyridoxine-5'-beta-D-glucoside hydrolase activity
Rattus norvegicus
lactose
inhibition of pyridoxine-5'-beta-D-glucoside hydrolase activity, competitive
Rattus norvegicus
maltose
inhibition of pyridoxine-5'-beta-D-glucoside hydrolase activity, inhibition is reduced to 60-75% by addition of 1-deoxynojirimycin
Rattus norvegicus
additional information
no inhibition by pyridoxine, trehalose, and sucrose
Rattus norvegicus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
56
-
lactose
pH 6.0, 37°C
Rattus norvegicus
64
-
beta-D-glucose
pH 6.0, 37°C
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics
Rattus norvegicus
1
-
pyridoxine-5'-beta-D-glucoside
pH 6.0, 37°C
Rattus norvegicus
16
-
lactose
pH 6.0, 37°C
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
brush border
-
Rattus norvegicus
5903
-
cytosol
-
Rattus norvegicus
5829
-
membrane
-
Rattus norvegicus
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
phloretin + H2O
Rattus norvegicus
-
phloretate + phloroglucinol
-
-
?
pyridoxine-5'-beta-D-glucoside + H2O
Rattus norvegicus
enzyme might play an important role in providing the bioavailability of pyridoxine-5'-beta-D-glucoside
pyridoxine + D-glucose
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
300fold from intestine brushh border membrane
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mucosa
-
Rattus norvegicus
-
small intestine
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
lactose + H2O
-
656061
Rattus norvegicus
beta-D-galactose + beta-D-glucose
-
-
-
?
additional information
the enzyme preparation also shows maltase-glucoamylase activity, EC 3.2.1.20, at 3.8 U/mg
656061
Rattus norvegicus
?
-
-
-
-
phloretin + H2O
-
656061
Rattus norvegicus
phloretate + phloroglucinol
-
-
-
?
pyridoxine-5'-beta-D-glucoside + H2O
-
656061
Rattus norvegicus
pyridoxine + D-glucose
-
-
-
?
pyridoxine-5'-beta-D-glucoside + H2O
enzyme might play an important role in providing the bioavailability of pyridoxine-5'-beta-D-glucoside
656061
Rattus norvegicus
pyridoxine + D-glucose
-
-
-
?
pyridoxine-5'-beta-D-glucoside + lactose
-
656061
Rattus norvegicus
pyridoxine + beta-D-glucose + pyridoxine disaccharide
product identification, product is formed by a pyridoxine molecule with the 5'-beta-gkucoside and another glucose or galactose moiety
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 135000-140000, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1
-
pyridoxine-5'-beta-D-glucoside
pH 6.0, 37°C
Rattus norvegicus
47
-
lactose
pH 6.0, 37°C
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
assay at
Rattus norvegicus
Other publictions for EC 3.7.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750528
Han
Discovery and structural anal ...
Mycobacteroides abscessus 103, Mycobacteroides abscessus
FEBS J.
286
1959-1971
2019
-
-
1
1
13
-
1
6
-
1
-
4
-
8
-
-
1
-
-
-
-
-
4
-
-
-
-
6
-
-
-
-
2
-
-
-
-
1
-
1
13
-
-
1
2
6
-
1
-
4
-
-
-
1
-
-
-
-
4
-
-
-
-
6
-
-
-
-
-
-
-
-
5
5
732550
Zhou
A comparative study on the met ...
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
Molecules
19
177-203
2014
-
-
-
-
-
-
1
-
1
-
-
10
-
2
-
-
-
-
-
1
-
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
10
-
-
-
-
-
1
-
-
13
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
735304
Frank
-
Mutational analysis of the C-C ...
Topics Catal.
57
376-384
2014
-
-
1
-
3
-
-
-
-
1
2
1
-
1
-
-
1
-
-
-
-
-
3
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
1
2
1
-
-
-
1
-
-
-
-
3
1
1
-
-
-
1
-
-
-
-
2
2
-
-
-
752294
Frank
-
Mutational analysis of the C- ...
Top. Catal.
57
376-384
2014
-
-
1
1
-
-
-
-
-
1
-
1
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697434
Lehtimaeki
Adult-type hypolactasia is not ...
Homo sapiens
Clin. Sci.
115
265-271
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
699107
Delacour
Loss of galectin-3 impairs mem ...
Mus musculus 129Sv, Mus musculus
J. Cell Sci.
121
458-465
2008
-
-
1
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
701338
Cramm-Behrens
Apical cargo traverses endosom ...
Canis lupus familiaris
Traffic
9
2206-2220
2008
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685892
Bulhoes
Correlation between lactose ab ...
Homo sapiens
Braz. J. Med. Biol. Res.
40
1441-1446
2007
-
1
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687838
Piepoli
Genotyping of the lactase-phlo ...
Homo sapiens
J. Biomol. Screen.
12
733-739
2007
-
1
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673123
Bodlaj
Genotyping of the lactase-phlo ...
Homo sapiens
Clin. Chem.
52
148-151
2006
-
1
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675574
Kuranuki
Dietary sucrose enhances intes ...
Rattus norvegicus
J. Nutr. Sci. Vitaminol.
52
347-351
2006
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675975
Bosse
Gata4 is essential for the mai ...
Mus musculus
Mol. Cell. Biol.
26
9060-9070
2006
3
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654284
Schoefer
Cloning and Expression of a Ph ...
, wK1
Appl. Environ. Microbiol.
70
6131-6137
2004
-
-
1
-
-
-
9
1
-
1
3
3
-
5
-
-
1
1
-
-
2
-
11
2
1
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
9
-
1
-
1
3
3
-
-
-
1
-
-
2
-
11
2
1
-
-
1
1
-
-
-
-
2
2
-
-
-
671823
Tseung
Partial amino acid sequence an ...
Homo sapiens, Oryctolagus cuniculus
Biochem. J.
380
211-218
2004
-
2
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675587
Verhave
Regulatory regions in the rat ...
Rattus norvegicus
J. Pediatr. Gastroenterol. Nutr.
39
275-285
2004
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656061
Mackey
Enzymatic hydrolysis of pyrido ...
Rattus norvegicus
J. Biol. Chem.
277
26858-26864
2002
-
-
-
-
-
-
6
3
3
-
-
2
-
2
-
-
1
1
-
2
-
-
6
1
1
-
-
2
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
6
2
3
3
-
-
2
-
-
-
1
-
2
-
-
6
1
1
-
-
2
1
-
-
-
-
-
-
-
-
-
656110
Jacob
Prosequence of human lactase-p ...
Homo sapiens
J. Biol. Chem.
277
8217-8225
2002
-
-
1
-
-
-
-
-
2
-
-
-
-
1
-
2
1
1
-
-
1
-
3
1
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
1
-
-
1
-
3
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
208760
Ramaswamy
Lactase-phlorizin hydrolase co ...
Platyrrhini
Biochim. Biophys. Acta
403
446-455
1975
-
-
-
-
-
-
2
3
-
-
-
-
-
1
-
-
1
-
-
1
1
-
12
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
1
-
1
1
-
12
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
208761
Birkenmeier
Enzymatic properties of rat la ...
Rattus norvegicus
Biochim. Biophys. Acta
350
100-112
1974
7
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
2
1
-
-
1
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
2
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
289291
Minamikawa
An inducible hydrolase from As ...
Aspergillus niger
Biochem. J.
116
889-897
1970
-
-
-
-
-
-
12
2
-
-
-
-
-
1
-
-
1
-
-
1
1
-
12
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
12
-
2
-
-
-
-
-
-
-
1
-
1
1
-
12
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
289292
Chatterjee
Metabolism of phloridzin by Er ...
Pantoea agglomerans, Pantoea agglomerans Y46
J. Bacteriol.
100
594-600
1969
-
-
-
-
-
-
2
1
-
-
-
-
-
3
-
-
1
-
-
-
1
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-