BRENDA - Enzyme Database show
show all sequences of 3.7.1.4

An inducible hydrolase from Aspergillus niger, acting on carbon-carbon bonds, for phlorrhizin and other C-acylated phenols

Minamikawa, T.; Jayasankar, N.P.; Bohm, B.A.; Taylor, I.E.P.; Towers, G.H.N.; Biochem. J. 116, 889-897 (1970)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Ag+
0.02 mM
Aspergillus niger
Al2(SO4)3
1 mM
Aspergillus niger
Cd2+
1 mM
Aspergillus niger
Co2+
1 mM
Aspergillus niger
Cu2+
1 mM
Aspergillus niger
Fe3+
1 mM
Aspergillus niger
Hg2+
0.02 mM
Aspergillus niger
L-cysteine
in presence of p-chloromercuribenzoate, 0.05 mM
Aspergillus niger
Na2MoO4
1 mM
Aspergillus niger
Na2WO4
1 mM
Aspergillus niger
p-chloromercuribenzoate
0.05 mM
Aspergillus niger
Zn2+
0.2 mM
Aspergillus niger
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.15
-
2-chloro-1-(3-methylphenyl)ethanone
-
Aspergillus niger
0.3
0.4
phloretin
-
Aspergillus niger
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus niger
-
-
-
Purification (Commentary)
Commentary
Organism
-
Aspergillus niger
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mycelial felt
-
Aspergillus niger
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.6
-
-
Aspergillus niger
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2',3,4',6'-tetrahydroxy-4-methoxydihydrochalcone + H2O
-
289291
Aspergillus niger
phloroglucinol + ?
-
289291
Aspergillus niger
?
2',3,4',6'-tetrahydroxy-dihydrochalcone + H2O
-
289291
Aspergillus niger
phloroglucinol + ?
-
289291
Aspergillus niger
?
2',3,4,4',6'-pentahydroxy-dihydrochalcone 2'-beta-D-glucoside + H2O
-
289291
Aspergillus niger
phloroglucinol beta-D-glucoside + 3-(3,4-dihydroxyphenyl)propanoic acid
-
289291
Aspergillus niger
?
2',3,4,4',6'-tetrahydroxy-dihydrochalcone + H2O
-
289291
Aspergillus niger
phloroglucinol + ?
-
289291
Aspergillus niger
?
2',4'-dihydroxy-4-methoxydihydrochalcone + H2O
-
289291
Aspergillus niger
resorcinol + ?
-
289291
Aspergillus niger
?
2',4'-dihydroxypropiophenone + H2O
-
289291
Aspergillus niger
resorcinol
-
289291
Aspergillus niger
?
2',4,4'-trihydroxyhydrochalcone
-
289291
Aspergillus niger
?
-
-
-
?
2',6'-dihydroxy-4,4'-dimethoxydihydrochalcone + H2O
-
289291
Aspergillus niger
3-methoxyresorcinol + ?
-
289291
Aspergillus niger
?
3'-methylchloracetophenone + H2O
-
289291
Aspergillus niger
?
-
-
-
?
chloracetophenone + H2O
-
289291
Aspergillus niger
?
-
-
-
?
phloretin + H2O
-
289291
Aspergillus niger
phloretate + phloroglucinol
-
289291
Aspergillus niger
?
phlorhizin + H2O
-
289291
Aspergillus niger
3-(4-hydroxyphenyl)propanoate + arbutin
-
289291
Aspergillus niger
?
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
-20
-
after 4 weeks loss of 20% activity
Aspergillus niger
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.6
-
-
Aspergillus niger
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ag+
0.02 mM
Aspergillus niger
Al2(SO4)3
1 mM
Aspergillus niger
Cd2+
1 mM
Aspergillus niger
Co2+
1 mM
Aspergillus niger
Cu2+
1 mM
Aspergillus niger
Fe3+
1 mM
Aspergillus niger
Hg2+
0.02 mM
Aspergillus niger
L-cysteine
in presence of p-chloromercuribenzoate, 0.05 mM
Aspergillus niger
Na2MoO4
1 mM
Aspergillus niger
Na2WO4
1 mM
Aspergillus niger
p-chloromercuribenzoate
0.05 mM
Aspergillus niger
Zn2+
0.2 mM
Aspergillus niger
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.15
-
2-chloro-1-(3-methylphenyl)ethanone
-
Aspergillus niger
0.3
0.4
phloretin
-
Aspergillus niger
Purification (Commentary) (protein specific)
Commentary
Organism
-
Aspergillus niger
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mycelial felt
-
Aspergillus niger
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.6
-
-
Aspergillus niger
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2',3,4',6'-tetrahydroxy-4-methoxydihydrochalcone + H2O
-
289291
Aspergillus niger
phloroglucinol + ?
-
289291
Aspergillus niger
?
2',3,4',6'-tetrahydroxy-dihydrochalcone + H2O
-
289291
Aspergillus niger
phloroglucinol + ?
-
289291
Aspergillus niger
?
2',3,4,4',6'-pentahydroxy-dihydrochalcone 2'-beta-D-glucoside + H2O
-
289291
Aspergillus niger
phloroglucinol beta-D-glucoside + 3-(3,4-dihydroxyphenyl)propanoic acid
-
289291
Aspergillus niger
?
2',3,4,4',6'-tetrahydroxy-dihydrochalcone + H2O
-
289291
Aspergillus niger
phloroglucinol + ?
-
289291
Aspergillus niger
?
2',4'-dihydroxy-4-methoxydihydrochalcone + H2O
-
289291
Aspergillus niger
resorcinol + ?
-
289291
Aspergillus niger
?
2',4'-dihydroxypropiophenone + H2O
-
289291
Aspergillus niger
resorcinol
-
289291
Aspergillus niger
?
2',4,4'-trihydroxyhydrochalcone
-
289291
Aspergillus niger
?
-
-
-
?
2',6'-dihydroxy-4,4'-dimethoxydihydrochalcone + H2O
-
289291
Aspergillus niger
3-methoxyresorcinol + ?
-
289291
Aspergillus niger
?
3'-methylchloracetophenone + H2O
-
289291
Aspergillus niger
?
-
-
-
?
chloracetophenone + H2O
-
289291
Aspergillus niger
?
-
-
-
?
phloretin + H2O
-
289291
Aspergillus niger
phloretate + phloroglucinol
-
289291
Aspergillus niger
?
phlorhizin + H2O
-
289291
Aspergillus niger
3-(4-hydroxyphenyl)propanoate + arbutin
-
289291
Aspergillus niger
?
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
-20
-
after 4 weeks loss of 20% activity
Aspergillus niger
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.6
-
-
Aspergillus niger
Other publictions for EC 3.7.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750528
Han
Discovery and structural anal ...
Mycobacteroides abscessus 103, Mycobacteroides abscessus
FEBS J.
286
1959-1971
2019
-
-
1
1
13
-
1
6
-
1
-
4
-
8
-
-
1
-
-
-
-
-
4
-
-
-
-
6
-
-
-
-
2
-
-
-
-
1
-
1
13
-
-
1
2
6
-
1
-
4
-
-
-
1
-
-
-
-
4
-
-
-
-
6
-
-
-
-
-
-
-
-
5
5
732550
Zhou
A comparative study on the met ...
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
Molecules
19
177-203
2014
-
-
-
-
-
-
1
-
1
-
-
10
-
2
-
-
-
-
-
1
-
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
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1
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-
10
-
-
-
-
-
1
-
-
13
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
735304
Frank
-
Mutational analysis of the C-C ...
Topics Catal.
57
376-384
2014
-
-
1
-
3
-
-
-
-
1
2
1
-
1
-
-
1
-
-
-
-
-
3
1
1
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1
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1
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3
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1
2
1
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1
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3
1
1
-
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-
1
-
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2
2
-
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752294
Frank
-
Mutational analysis of the C- ...
Top. Catal.
57
376-384
2014
-
-
1
1
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1
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1
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697434
Lehtimaeki
Adult-type hypolactasia is not ...
Homo sapiens
Clin. Sci.
115
265-271
2008
-
-
1
-
-
-
-
-
-
-
-
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1
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699107
Delacour
Loss of galectin-3 impairs mem ...
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121
458-465
2008
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1
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2
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2
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1
1
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701338
Cramm-Behrens
Apical cargo traverses endosom ...
Canis lupus familiaris
Traffic
9
2206-2220
2008
-
-
-
-
-
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-
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2
-
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1
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1
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1
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685892
Bulhoes
Correlation between lactose ab ...
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40
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2007
-
1
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1
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1
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1
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1
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2
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1
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1
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1
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2
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687838
Piepoli
Genotyping of the lactase-phlo ...
Homo sapiens
J. Biomol. Screen.
12
733-739
2007
-
1
-
-
1
-
-
-
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1
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1
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-
-
1
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2
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1
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1
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2
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673123
Bodlaj
Genotyping of the lactase-phlo ...
Homo sapiens
Clin. Chem.
52
148-151
2006
-
1
-
-
1
-
-
-
-
-
-
1
-
1
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1
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3
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1
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1
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1
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1
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3
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675574
Kuranuki
Dietary sucrose enhances intes ...
Rattus norvegicus
J. Nutr. Sci. Vitaminol.
52
347-351
2006
1
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1
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1
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2
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2
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2
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675975
Bosse
Gata4 is essential for the mai ...
Mus musculus
Mol. Cell. Biol.
26
9060-9070
2006
3
-
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1
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1
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1
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654284
Schoefer
Cloning and Expression of a Ph ...
, wK1
Appl. Environ. Microbiol.
70
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-
-
1
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-
-
9
1
-
1
3
3
-
5
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-
1
1
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-
2
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11
2
1
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-
1
1
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-
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1
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9
-
1
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1
3
3
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1
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2
-
11
2
1
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1
1
-
-
-
-
2
2
-
-
-
671823
Tseung
Partial amino acid sequence an ...
Homo sapiens, Oryctolagus cuniculus
Biochem. J.
380
211-218
2004
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2
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675587
Verhave
Regulatory regions in the rat ...
Rattus norvegicus
J. Pediatr. Gastroenterol. Nutr.
39
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2004
-
1
1
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1
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656061
Mackey
Enzymatic hydrolysis of pyrido ...
Rattus norvegicus
J. Biol. Chem.
277
26858-26864
2002
-
-
-
-
-
-
6
3
3
-
-
2
-
2
-
-
1
1
-
2
-
-
6
1
1
-
-
2
1
-
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-
2
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-
-
-
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6
2
3
3
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2
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1
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2
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-
6
1
1
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2
1
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656110
Jacob
Prosequence of human lactase-p ...
Homo sapiens
J. Biol. Chem.
277
8217-8225
2002
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1
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2
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1
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1
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1
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1
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2
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2
1
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1
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3
1
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1
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208760
Ramaswamy
Lactase-phlorizin hydrolase co ...
Platyrrhini
Biochim. Biophys. Acta
403
446-455
1975
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2
3
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1
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1
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12
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1
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12
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1
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208761
Birkenmeier
Enzymatic properties of rat la ...
Rattus norvegicus
Biochim. Biophys. Acta
350
100-112
1974
7
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7
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1
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1
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289291
Minamikawa
An inducible hydrolase from As ...
Aspergillus niger
Biochem. J.
116
889-897
1970
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12
2
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12
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12
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1
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12
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1
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1
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289292
Chatterjee
Metabolism of phloridzin by Er ...
Pantoea agglomerans, Pantoea agglomerans Y46
J. Bacteriol.
100
594-600
1969
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3
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