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Literature summary for 3.7.1.4 extracted from

  • Ramaswamy, S.; Radhakrishnan, A.N.
    Lactase-phlorizin hydrolase complex from monkey small intestine. Purification, properties and evidence for two catalytic sites (1975), Biochim. Biophys. Acta, 403, 446-455.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
brain cerebroside competitive inhibition Platyrrhini
phlorizin competitive inhibition Platyrrhini

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
phlorizin
-
Platyrrhini
2.78
-
cellobiose
-
Platyrrhini
21.8
-
lactose
-
Platyrrhini

Organism

Organism UniProt Comment Textmining
Platyrrhini
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Platyrrhini

Source Tissue

Source Tissue Comment Organism Textmining
small intestine
-
Platyrrhini
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.45
-
-
Platyrrhini

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-methyl D-glucoside + H2O
-
Platyrrhini ?
-
?
cellobiose + H2O
-
Platyrrhini 2 D-glucose
-
?
cerebrosides + H2O
-
Platyrrhini ?
-
?
lactose + H2O
-
Platyrrhini D-galactose + D-glucose
-
?
N-palmitoyldihydroglucosylceramide + H2O
-
Platyrrhini ?
-
?
o-nitrophenyl-beta-D-galactoside + H2O
-
Platyrrhini o-nitrophenol + beta-D-galactose
-
?
o-nitrophenyl-beta-D-glucoside + H2O
-
Platyrrhini o-nitrophenol + beta-D-glucose
-
?
p-nitrophenyl-beta-D-galactoside + H2O
-
Platyrrhini p-nitrophenol + beta-D-galactose
-
?
p-nitrophenyl-beta-D-glucoside + H2O
-
Platyrrhini p-nitrophenol + beta-D-glucose
-
?
phloretin + H2O
-
Platyrrhini phloretate + phloroglucinol
-
?
phlorhizin + H2O
-
Platyrrhini 3-(4-hydroxyphenyl)propanoate + arbutin
-
?
salicin + H2O
-
Platyrrhini ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
-
Platyrrhini