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Literature summary for 3.7.1.3 extracted from

  • Kumar, S.; Gawandi, V.B.; Capito, N.; Phillips, R.S.
    Substituent effects on the reaction of beta-benzoylalanines with Pseudomonas fluorescens kynureninase (2010), Biochemistry, 49, 7913-7919.
    View publication on PubMed
Search for more literature for 3.7.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH-5alpha cells Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-kynurenine + H2O Pseudomonas fluorescens
-
 anthranilate + L-alanine
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-4-(2-chlorophenyl)-4-oxobutanoic acid + H2O
-
 Pseudomonas fluorescens ?
-
 ?
2-amino-4-(2-methoxyphenyl)-4-oxobutanoic acid + H2O
-
 Pseudomonas fluorescens ?
-
 ?
2-amino-4-(4-methoxyphenyl)-4-oxobutanoic acid + H2O
-
 Pseudomonas fluorescens ?
-
 ?
2-fluorobenzoyl-DL-alanine + H2O
-
 Pseudomonas fluorescens 2-fluorobenzoate + beta-alanine
-
 ?
beta-(4-chlorobenzoyl)-DL-alanine + H2O
-
 Pseudomonas fluorescens 4-chlorobenzoate + beta-alanine
-
 ?
beta-(4-methylbenzoyl)-DL-alanine + H2O
-
 Pseudomonas fluorescens 4-methylbenzoate + beta-alanine
-
 ?
beta-(4-trifluoromethylbenzoyl)-DL-alanine + H2O
-
 Pseudomonas fluorescens 4-trifluoromethylbenzoate + beta-alanine
-
 ?
beta-benzoyl-L-alanine + H2O good substrate Pseudomonas fluorescens benzoate + L-alanine
-
 ?
L-kynurenine + H2O
-
 Pseudomonas fluorescens anthranilate + L-alanine
-
 ?

Synonyms

Synonyms Comment Organism
Kynase
-
 Pseudomonas fluorescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
 beta-(4-trifluoromethylbenzoyl)-DL-alanine at 37°C in 0.04 M potassium phosphate, pH 7.8 Pseudomonas fluorescens
0.05
-
 2-amino-4-(2-methoxyphenyl)-4-oxobutanoic acid at 37°C in 0.04 M potassium phosphate, pH 7.8 Pseudomonas fluorescens
0.06
-
 2-fluorobenzoyl-DL-alanine at 37°C in 0.04 M potassium phosphate, pH 7.8 Pseudomonas fluorescens
0.08
-
 2-amino-4-(2-chlorophenyl)-4-oxobutanoic acid at 37°C in 0.04 M potassium phosphate, pH 7.8 Pseudomonas fluorescens
0.38
-
 2-amino-4-(4-methoxyphenyl)-4-oxobutanoic acid at 37°C in 0.04 M potassium phosphate, pH 7.8 Pseudomonas fluorescens
0.91
-
 beta-(4-methylbenzoyl)-DL-alanine at 37°C in 0.04 M potassium phosphate, pH 7.8 Pseudomonas fluorescens
1.83
-
 beta-(4-chlorobenzoyl)-DL-alanine at 37°C in 0.04 M potassium phosphate, pH 7.8 Pseudomonas fluorescens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Pseudomonas fluorescens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.019
-
 beta-(4-trifluoromethylbenzoyl)-DL-alanine at 37°C in 0.04 M potassium phosphate, pH 7.8 Pseudomonas fluorescens
1.4
-
 2-amino-4-(2-methoxyphenyl)-4-oxobutanoic acid at 37°C in 0.04 M potassium phosphate, pH 7.8 Pseudomonas fluorescens
1.6
-
 2-amino-4-(4-methoxyphenyl)-4-oxobutanoic acid at 37°C in 0.04 M potassium phosphate, pH 7.8 Pseudomonas fluorescens
1.7
-
 2-fluorobenzoyl-DL-alanine at 37°C in 0.04 M potassium phosphate, pH 7.8 Pseudomonas fluorescens
12
-
 2-amino-4-(2-chlorophenyl)-4-oxobutanoic acid at 37°C in 0.04 M potassium phosphate, pH 7.8 Pseudomonas fluorescens
18
-
 beta-(4-chlorobenzoyl)-DL-alanine at 37°C in 0.04 M potassium phosphate, pH 7.8 Pseudomonas fluorescens
28
-
 beta-(4-methylbenzoyl)-DL-alanine at 37°C in 0.04 M potassium phosphate, pH 7.8 Pseudomonas fluorescens