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Literature summary for 3.7.1.3 extracted from

  • Gawandi, V.B.; Liskey, D.; Lima, S.; Phillips, R.S.
    Reaction of Pseudomonas fluorescens kynureninase with beta-benzoyl-L-alanine: Detection of a new reaction intermediate and a change in rate-determining step (2004), Biochemistry, 43, 3230-3237.
    View publication on PubMed
Search for more literature for 3.7.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas fluorescens
into vector pTZKYN and transformed in Escherichia coli XL-1 Blue cells Pseudomonas fluorescens

Inhibitors

Inhibitors Comment Organism Structure
(2S)-2-amino-4-oxo-4-phenylbutanoic acid competitive inhibitor of the reaction of L-kynurenine with the enzyme Pseudomonas fluorescens
beta-benzoyl-L-alanine competitive inhibition, at 25ºC, pH 7.8 Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 beta-benzoyl-L-alanine at 25ºC, pH 7.8 Pseudomonas fluorescens
0.03
-
 L-kynurenine at 25ºC, pH 7.8 Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-kynurenine + H2O Pseudomonas fluorescens L-tryptophan metabolism anthranilate + L-alanine
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no modification
-
 Pseudomonas fluorescens

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas fluorescens
by chromatography on phenyl-Sepharose Pseudomonas fluorescens

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Pseudomonas fluorescens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 19 U/mg protein Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-2-amino-4-oxo-4-phenylbutanoic acid + H2O the rate-determining step is Cbeta-Cgamma bond cleavage Pseudomonas fluorescens benzoate + L-alanine
-
 ?
beta-benzoyl-L-alanine + H2O
-
 Pseudomonas fluorescens benzoate + L-alanine
-
 ?
L-kynurenine + H2O
-
 Pseudomonas fluorescens anthranilate + L-alanine
-
 ?
L-kynurenine + H2O L-tryptophan metabolism Pseudomonas fluorescens anthranilate + L-alanine
-
 ?
L-kynurenine + H2O the rate-determining step is to be deprotonation of the pyruvate-ketimine intermediate Pseudomonas fluorescens anthranilate + L-alanine
-
 ?

Synonyms

Synonyms Comment Organism
L-kynurenine hydrolase
-
 Pseudomonas fluorescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.7
-
 beta-benzoyl-L-alanine
-
 Pseudomonas fluorescens
0.7
-
 (2S)-2-amino-4-oxo-4-phenylbutanoic acid at pH 7.8 Pseudomonas fluorescens
16
-
 L-kynurenine
-
 Pseudomonas fluorescens
16
-
 L-kynurenine at pH 7.8 Pseudomonas fluorescens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Pseudomonas fluorescens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.01
-
 beta-benzoyl-L-alanine at 25ºC, pH 7.8 Pseudomonas fluorescens
0.01
-
 (2S)-2-amino-4-oxo-4-phenylbutanoic acid reaction of L-kynurenine with the enzyme Pseudomonas fluorescens